Alternative polyadenylation sites of human endothelial nitric oxide synthase mRNA

Hideshi Ihara, Hiroyasu Tsutsuki, Tomoaki Ida, Shunji Kozaki, Shingo Tsuyama, Joel Moss

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The mRNA 3′-untranslated region (3′-UTR) has been shown to have important roles in the regulation of mRNA function. In this study, we investigated the human endothelial nitric oxide synthase (eNOS) 3′-UTR to evaluate its potential regulatory role. 3′-RACE analysis revealed that the human eNOS mRNA has multiple alternative polyadenylation sites. Apart from the proximal site (418 bp downstream of the stop codon), we identified two additional distal sites approximately 770 and 1478 bp downstream of the stop codon. In addition, Northern analysis showed that the usage of these sites differed among human tissues. Further, amounts of these eNOS mRNAs were changed during growth of cultured human aortic endothelial cells; mRNAs with long 3′-UTRs decreased more rapidly than total mRNA, as cells approached confluency. Thus, the 3′-UTRs of human eNOS results from alternative polyadenylation sites and differ across tissues and during cell growth.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalBiochemical and biophysical research communications
Volume363
Issue number1
DOIs
Publication statusPublished - 2007 Nov 9
Externally publishedYes

Keywords

  • 3′-Untranslated region
  • Alternative polyadenylation sites
  • eNOS

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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