Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Takashi Okitsu; Take Matsuyama; Takahiro Yamashit; Toru Ishizuk; Hiromu Yawo; Yasushi Imamoto; Yoshinori Shichid; Akimori Wada

doi:10.1248/cpb.c17-00054

Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Takashi Okitsu, Take Matsuyama, Takahiro Yamashit, Toru Ishizuk, Hiromu Yawo, Yasushi Imamoto, Yoshinori Shichid, Akimori Wada

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.

Original language	English
Pages (from-to)	356-358
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	65
Issue number	4
DOIs	https://doi.org/10.1248/cpb.c17-00054
Publication status	Published - 2017

Keywords

Channelrhodopsin
Chromophore
Neuroscience
Optogenetics
Red-shift

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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10.1248/cpb.c17-00054

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abstract = "Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.",

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AU - Okitsu, Takashi

AU - Matsuyama, Take

AU - Yamashit, Takahiro

AU - Ishizuk, Toru

AU - Yawo, Hiromu

AU - Imamoto, Yasushi

AU - Shichid, Yoshinori

AU - Wada, Akimori

PY - 2017

Y1 - 2017

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AB - Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.

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KW - Chromophore

KW - Neuroscience

KW - Optogenetics

KW - Red-shift

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Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Abstract

Keywords

ASJC Scopus subject areas

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