Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Takashi Okitsu, Take Matsuyama, Takahiro Yamashit, Toru Ishizuk, Hiromu Yawo, Yasushi Imamoto, Yoshinori Shichid, Akimori Wada

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.

    Original languageEnglish
    Pages (from-to)356-358
    Number of pages3
    JournalChemical and Pharmaceutical Bulletin
    Volume65
    Issue number4
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Channelrhodopsin
    • Chromophore
    • Neuroscience
    • Optogenetics
    • Red-shift

    ASJC Scopus subject areas

    • Chemistry(all)
    • Drug Discovery

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