TY - JOUR
T1 - Altered profile of endogeneous steroids in the urine of patients with prolactinoma
AU - Lee, Seon Hwa
AU - Nam, Su Youn
AU - Chung, Bong Chul
N1 - Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 1998/10
Y1 - 1998/10
N2 - Objective: The aim of this study was to investigate the possible effect of prolactin on the metabolism of androgens and estrogens in patients with prolactinoma. To accomplish this, prolactin, urinary androgen, and estrogen metabolite levels were determined. In order to indirectly evaluate the possible involvement of enzymes, the concentration ratios of precursor metabolite to product metabolite were also compared with controls. Methods: Urine samples were obtained from 27 female patients with prolactinoma (macro, micro, and idiopathic) and from 31 age-matched normal female subjects. Urinary metabolites of 21 androgens and corticoids and 20 estrogens were analyzed by a gas chromatography-mass spectrometry system. Results: In patients with prolactinoma, urinary 17-ketosteroids, and all estrogen metabolite concentrations were elevated. The ratios of Δ5/Δ4-steroids and 5β/5α-hydrogensteroids were higher in the patients with prolactinoma than in normal female controls. In addition, no significant differences between patients and controls were observed in the precursor metabolite to product metabolite ratios relating to estrogen metabolism. Conclusion: Our data suggests that enhanced PRL levels have a direct effect on urinary steroid secretion and metabolism, probably due to lowered activities of 5α-reductase and 3β-hydroxysteroid dehydrogenase in the patients with prolactinoma.
AB - Objective: The aim of this study was to investigate the possible effect of prolactin on the metabolism of androgens and estrogens in patients with prolactinoma. To accomplish this, prolactin, urinary androgen, and estrogen metabolite levels were determined. In order to indirectly evaluate the possible involvement of enzymes, the concentration ratios of precursor metabolite to product metabolite were also compared with controls. Methods: Urine samples were obtained from 27 female patients with prolactinoma (macro, micro, and idiopathic) and from 31 age-matched normal female subjects. Urinary metabolites of 21 androgens and corticoids and 20 estrogens were analyzed by a gas chromatography-mass spectrometry system. Results: In patients with prolactinoma, urinary 17-ketosteroids, and all estrogen metabolite concentrations were elevated. The ratios of Δ5/Δ4-steroids and 5β/5α-hydrogensteroids were higher in the patients with prolactinoma than in normal female controls. In addition, no significant differences between patients and controls were observed in the precursor metabolite to product metabolite ratios relating to estrogen metabolism. Conclusion: Our data suggests that enhanced PRL levels have a direct effect on urinary steroid secretion and metabolism, probably due to lowered activities of 5α-reductase and 3β-hydroxysteroid dehydrogenase in the patients with prolactinoma.
KW - Androgen
KW - Estrogen
KW - Gas chromatography-mass spectrometry
KW - Prolactinoma
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U2 - 10.1016/S0009-9120(98)00063-0
DO - 10.1016/S0009-9120(98)00063-0
M3 - Article
C2 - 9812172
AN - SCOPUS:0032176688
VL - 31
SP - 529
EP - 535
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 7
ER -