Altered magnetic resonance images of brain and social behaviors of hatchling, and expression of thyroid hormone receptor βmRNA in cerebellum of embryos after Methimazole administration

Gen Haba, Hidekazu Nishigori, Makoto Sasaki, Koujiro Tohyama, Kohsuke Kudo, Yutaka Matsumura, Toru Sugiyama, Keisuke Kagami, Yu Tezuka, Atsushi Sanbe, Hideo Nishigori

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Rationale and objectives: The effects of low thyroid hormone level during embryogenesis on MRI of the brain and social behaviors of hatchlings were examined using "fertilized hen's egg-embryo-chick" system. Methods and results: Control and hatchlings treated with methimazole (20 μmol/egg), which hatched 3 days later than controls were examined. The results are as follows: 1. The MRI examination of the midsagittal section of the brain on hatch day showed that the sizes, by T1- and ADC values by diffusion-weighted images, of the optic lobe and cerebellum of the MMI-hatchlings were significantly bigger than those of the controls. 2. The social behaviors on post-hatch day 3 were based on the following tests: (a) Aggregation test: The speed of four chicks, individually isolated by cardboard barriers in a box, to make a group upon the removal of barriers. (b) Belongingness tests: The speed of a chick isolated at a corner to join the group of three chicks placed at the opposite corner. (c) Vocalization test: The number of decibel produced by a chick isolated at a corner using a sound meter. These tests demonstrated that MMI-hatchlings took longer times and had weaker vocalization than the controls, significantly. 3. Upregulation of THRβ mRNA after MMI treatment suggested that THR was necessary for cerebellum development. Conclusions: The MMI exposure during the last week of embryogenesis possibly delayed the myelination of certain brain regions and impaired the social behaviors of hatchlings. The chick embryos can be easily induced with hypothyroidism without maternal influences, and the hatchling's behaviors were analyzed using a video camera. The present method will be useful for assessing the effects of unfavorable influences during embryogenesis on social behaviors in later life.

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalPsychopharmacology
Volume231
Issue number1
DOIs
Publication statusPublished - 2014 Jan

Keywords

  • Aggregation
  • Cerebellum
  • Chick
  • Inorganic pyrophosphatase
  • MRI
  • Methimazole
  • Secernin
  • Social behaviors
  • Thyroid hormone receptor
  • Vocalization

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Altered magnetic resonance images of brain and social behaviors of hatchling, and expression of thyroid hormone receptor βmRNA in cerebellum of embryos after Methimazole administration'. Together they form a unique fingerprint.

Cite this