Altered gene expressions involved in energy expenditure in 5-HT2C receptor mutant mice

Katsunori Nonogaki, Riaz A. Memon, Carl Grunfeld, Kenneth R. Feingold, Laurence H. Tecott

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Mice with a targeted null mutation of the serotonin 5-HT2C receptor gene exhibit hyperphagia that leads to a late-onset obesity. Here we show that oxygen consumption was decreased in fed and fasted obese mutants. No phenotypic differences were observed in uncoupling protein-1 (UCP-1) mRNA levels in brown adipose tissues and UCP-3 mRNA in skeletal muscle. UCP-2 mRNA levels were significantly increased in white adipose tissue (4-fold) and skeletal muscle (47%) in older obese mutant mice, whereas UCP-2 mRNA in liver are significantly increased in both young lean (54% increase) and older obese (52% increase) mutant mice. In contrast, 5-HT2C receptor mutants displayed age-dependent decreases in β3-adrenergic receptor (β3-AR) mRNA levels in white adipose tissue, however, no such changes were observed in brown adipose tissue. These results indicate that a mutation of 5-HT2C receptor gene leads to a secondary decrease in β3-AR gene expression that is related to enhanced adiposity.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalBiochemical and biophysical research communications
Volume295
Issue number2
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Keywords

  • 5-HT receptor
  • Hyperphagia
  • Obesity
  • Oxygen consumption
  • Serotonin
  • UCP
  • β3-adrenergic

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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