Altered fractionation profiles of higher plant poly(A)-containing RNA by selective formation of a complex with a hydrophobic impurity

Hideki Takahashi, Takeshi Nitta

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Synthetic polyadenylate (poly(A)) which originally sedimented at 6-7 S in a sucrose density gradient did so at 15-19 S in the presence of phenol-extracted nucleic acids from higher plant tissues. After centrifugation, the input poly(A) was found to be insensitive to RNase T2 (EC 3.129.1), suggesting a newly-formed complex with a hydrophobic substance contaminating the nucleic acid preparation. The artifically-formed poly(A)-impurity complex migrated more slowly than unbound poly(A) during gel electrophoresis. The complex was retained rather effectively by an oligodeoxythymidylate (oligo-(dT))-cellulose column and a polyuridylate (poly(U))-glassfiber filter. However, a nitrocellulose membrane filter retained the poly(A)-complex to various extents depending on plant materials, suggesting different varieties of the poly(A)-binding impurity in the higher plant kingdom. Fractionation of mRNA by these ordinarily used procedures is suggested to be variously altered by complex formation with this hydrophobic impurity.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalPlant Science
Volume43
Issue number1
DOIs
Publication statusPublished - 1986 Jan 1
Externally publishedYes

Keywords

  • Poly(A)-containing RNA
  • contaminant
  • fractionation
  • hydrophobic bond
  • proteoglycan

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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