Abstract
Synthetic polyadenylate (poly(A)) which originally sedimented at 6-7 S in a sucrose density gradient did so at 15-19 S in the presence of phenol-extracted nucleic acids from higher plant tissues. After centrifugation, the input poly(A) was found to be insensitive to RNase T2 (EC 3.129.1), suggesting a newly-formed complex with a hydrophobic substance contaminating the nucleic acid preparation. The artifically-formed poly(A)-impurity complex migrated more slowly than unbound poly(A) during gel electrophoresis. The complex was retained rather effectively by an oligodeoxythymidylate (oligo-(dT))-cellulose column and a polyuridylate (poly(U))-glassfiber filter. However, a nitrocellulose membrane filter retained the poly(A)-complex to various extents depending on plant materials, suggesting different varieties of the poly(A)-binding impurity in the higher plant kingdom. Fractionation of mRNA by these ordinarily used procedures is suggested to be variously altered by complex formation with this hydrophobic impurity.
Original language | English |
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Pages (from-to) | 63-67 |
Number of pages | 5 |
Journal | Plant Science |
Volume | 43 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1986 Jan 1 |
Externally published | Yes |
Keywords
- Poly(A)-containing RNA
- contaminant
- fractionation
- hydrophobic bond
- proteoglycan
ASJC Scopus subject areas
- Genetics
- Agronomy and Crop Science
- Plant Science