Altered expression of retinoblastoma protein-interacting zinc finger gene, RIZ, in human leukaemia

Osamu Sasaki, Kuniaki Meguro, Yasuo Tohmiya, Tadao Funato, Shigeki Shibahara, Takeshi Sasaki

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

The retinoblastoma protein-interacting zinc finger gene (RIZ), a member of the nuclear protein methyl-transferase superfamily, is characterized by the presence of the N-terminal PR domain. The RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the PR (PRDI-BF1 and RIZ homologous) domain, RIZ2 lacks it. RIZ gene expression is altered in a variety of human cancers and RIZ1 is now considered to be a candidate tumour suppressor. To investigate the role of RIZ in leukaemogenesis, we analysed the differential expression of RIZ1 and RIZ2 by quantitative real-time reverse-transcription polymerase chain reaction assay. Our results showed that the expression of RIZ1 was significantly decreased in leukaemia cell lines (14 out of 17, 82%) and in patients with acute myeloblastic leukaemia (eight out of 14, 57%). In contrast, RIZ2 expression was increased in patients with acute lymphoblastic leukaemia (eight out of 11, 73%), compared with normal bone marrow cells. These findings indicate that suppression of RIZ1 expression or enhancement of RIZ2 expression may have an important role in leukaemogenesis.

Original languageEnglish
Pages (from-to)940-948
Number of pages9
JournalBritish Journal of Haematology
Volume119
Issue number4
DOIs
Publication statusPublished - 2002

Keywords

  • DNA binding protein
  • Human acute leukaemia
  • RIZ
  • Real-time RT-PCR
  • Tumour suppressor gene

ASJC Scopus subject areas

  • Hematology

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