TY - JOUR
T1 - Altered expression of retinoblastoma protein-interacting zinc finger gene, RIZ, in human leukaemia
AU - Sasaki, Osamu
AU - Meguro, Kuniaki
AU - Tohmiya, Yasuo
AU - Funato, Tadao
AU - Shibahara, Shigeki
AU - Sasaki, Takeshi
PY - 2002
Y1 - 2002
N2 - The retinoblastoma protein-interacting zinc finger gene (RIZ), a member of the nuclear protein methyl-transferase superfamily, is characterized by the presence of the N-terminal PR domain. The RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the PR (PRDI-BF1 and RIZ homologous) domain, RIZ2 lacks it. RIZ gene expression is altered in a variety of human cancers and RIZ1 is now considered to be a candidate tumour suppressor. To investigate the role of RIZ in leukaemogenesis, we analysed the differential expression of RIZ1 and RIZ2 by quantitative real-time reverse-transcription polymerase chain reaction assay. Our results showed that the expression of RIZ1 was significantly decreased in leukaemia cell lines (14 out of 17, 82%) and in patients with acute myeloblastic leukaemia (eight out of 14, 57%). In contrast, RIZ2 expression was increased in patients with acute lymphoblastic leukaemia (eight out of 11, 73%), compared with normal bone marrow cells. These findings indicate that suppression of RIZ1 expression or enhancement of RIZ2 expression may have an important role in leukaemogenesis.
AB - The retinoblastoma protein-interacting zinc finger gene (RIZ), a member of the nuclear protein methyl-transferase superfamily, is characterized by the presence of the N-terminal PR domain. The RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the PR (PRDI-BF1 and RIZ homologous) domain, RIZ2 lacks it. RIZ gene expression is altered in a variety of human cancers and RIZ1 is now considered to be a candidate tumour suppressor. To investigate the role of RIZ in leukaemogenesis, we analysed the differential expression of RIZ1 and RIZ2 by quantitative real-time reverse-transcription polymerase chain reaction assay. Our results showed that the expression of RIZ1 was significantly decreased in leukaemia cell lines (14 out of 17, 82%) and in patients with acute myeloblastic leukaemia (eight out of 14, 57%). In contrast, RIZ2 expression was increased in patients with acute lymphoblastic leukaemia (eight out of 11, 73%), compared with normal bone marrow cells. These findings indicate that suppression of RIZ1 expression or enhancement of RIZ2 expression may have an important role in leukaemogenesis.
KW - DNA binding protein
KW - Human acute leukaemia
KW - RIZ
KW - Real-time RT-PCR
KW - Tumour suppressor gene
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U2 - 10.1046/j.1365-2141.2002.03972.x
DO - 10.1046/j.1365-2141.2002.03972.x
M3 - Article
C2 - 12472571
AN - SCOPUS:0036454465
VL - 119
SP - 940
EP - 948
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 4
ER -