Alterations of Ca2+ currents and their sensitivities to Ca2+ antagonists during application and after deprivation of nerve growth factor

Katsutoshi Furukawa, Hiroshi Onodera, Kyuya Kogure, Norio Akaike

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The alterations in morphology and Ca2+ currents (ICa) were assessed in PC12 cells, which were (1) control (untreated), (2) treated with nerve growth factor (NGF), and (3) deprived of NGF. NGF treatment induced neuronal differentiation morphologically and increased the current density of ICa, and especially that of the ω-conotoxin (ω-CgTX)-sensitive component. NGF-deprived cells manifested morphological deterioration and did not exhibit remarkable changes in the current density of the total ICa and dihydropyridine components, whereas the ω-CgTX-sensitive ICa component was decreased significantly compared with that in NGF-treated cells. The results suggest that the total function of Ca2+ channels, including the dihydropyridine component, does not seem to be lost at the initial stage of neuronal deterioration, whereas ω-CgTX-sensitive Ca2+ channels lost their function early.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalNeuroscience Letters
Volume161
Issue number1
DOIs
Publication statusPublished - 1993 Oct 14

Keywords

  • Ca current
  • Nerve growth factor
  • PC12 cell
  • Programmed cell death

ASJC Scopus subject areas

  • Neuroscience(all)

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