Alteration of the Level of Protein Phosphatase 2C(IA) mRNA during the Course of Differentiation of Skeleral Muscle Cells

Susumu Ohishi, Takayasu Kobayashi, Takayuki Terasawa, Takashi Murakami, Masatoshi Onoda, Shinri Tamura, Susumu Ohishi, Takayuki Terasawa, Takashi Murakami, Masatoshi Onoda, Shigeru Tsuiki

Research output: Contribution to journalArticlepeer-review

Abstract

On Northern hybridization using the cDNA of type 2C protein phosphatase as a probe, substantial amount of the mRNA of type 2C protein phosphatase was detected in various organs of rats, suggesting that the type 2C protein phosphatase gene is a housekeeping gene. A relatively high level of the mRNA, however, was found in skeletal muscle compared with other organs. Similar results were obtained with the organs of mice. In addition, the mRNA level in C3H10T1/2 cells (embryonal mesenchymal cells of mice) was much lower than in mature skeletal muscle of mice. The mRNA level of type 2C protein phosphatase was enhanced in accordance with the differentiation of the C3H10T1/2 cells into myoblasts induced by the transfection of MyoD cDNA. These results suggest that type 2C protein phosphatase is related to the mechanism of differentiation of skeletal muscle cells.

Original languageEnglish
Pages (from-to)279-282
Number of pages4
JournalTohoku Journal of Experimental Medicine
Volume168
Issue number2
DOIs
Publication statusPublished - 1992

Keywords

  • protein phosphatase
  • skeletal muscle differentiaton

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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