Alteration in brain presenilin-1 mRNA expression in sporadic Alzheimer's disease

Kenji Isoe-Wada, Katsuya Urakami, Yosuke Wakutani, Yoshidi Adachi, Hiroyuki Arai, Hidetada Sasaki, Kenji Nakashima

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Expression of presenilin-1 (PS-1) mRNA in an alternative splicing at the 3' end of exon 3 was examined in brain tissue, lymphocytes and cultured skin fibroblasts using the RT-polymerase chain reaction (RT-PCR) method. We quantified the relative ratios of the densities of the long form of PS-1 mRNA, which contains a sequence encoding four amino acids (VRSQ, denoted as VRSQ+) to the short form, which lacks the VRSQ sequence (VRSQ-). The brain tissue of subjects with sporadic Alzheimer's disease (AD) had reduced levels of the VRSQ+ form of the PS-1 mRNA compared to a control group. No significant differences appeared in peripheral tissues, such as lymphocytes or cultured skin fibroblasts in AD with control subjects. Changes in the alternative splicing of exon 3 may be specific to the brain and may play an important role in the pathogenesis of sporadic AD.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalEuropean Journal of Neurology
Volume6
Issue number2
DOIs
Publication statusPublished - 1999

Keywords

  • Alternative splicing
  • Presenilin-1
  • Sporadic

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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