Alteration in aortic wall stiffness and accumulation of collagen during the prediabetic stage of type II diabetes mellitus in rats

Takahisa Noma, Katsufumi Mizushige, Li Yao, Yang Yu, Hideyasu Kiyomoto, Naohisa Hosomi, Shoji Kimura, Youichi Abe, Koji Ohmori, Hirohide Matsuo

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Aortic damage during the prediabetic stage of diabetes mellitus (DM) was investigated in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, as an animal model of type II DM. In 30 OLETF and 30 nonDM rats, an oral glucose tolerance test was performed at 10, 20 and 30 weeks of age. At 15 and 30 weeks, intravascular ultrasound images and aortic pressure were recorded and the stiffness parameter β was calculated. The aortic walls were excised at 5, 15 and 30 weeks for histopathology and the measurement of hydroxyproline. At 10 weeks, blood glucose (mg/dl) and insulin concentrations (ng/ml) of the OLETF rats (2h; 168±30 and 0.82±0.1.5) were significantly high (nonDM: 118±15; p=0.02 and 0.16±0.64; p=0.003). At the prediabetic stage (15 weeks), β in the OLETF rats (2.5±0.9) was larger than in nonDM rats (1.4±0.4; p=0.0006), and the collagen (hydroxyproline) content/dry weight (mg/g) of the aortic wall was significantly higher in OLETF (33.5±3.1) than in nonDM rats (28.7±3.5; p<.05). Histopathological examination showed that from 15 weeks of age the medial wall thickness increased gradually. In the prediabetic stage, collagen accumulation may contribute to impairment of aortic wall stiffness in the OLETF rats, which would accelerate the aging process in the aortic wall.

Original languageEnglish
Pages (from-to)988-993
Number of pages6
JournalJAPANESE CIRCULATION JOURNAL
Volume63
Issue number12
DOIs
Publication statusPublished - 1999 Dec

Keywords

  • Arteries
  • Collagen
  • Remodeling
  • Type II diabetes mellitus
  • Ultrasound

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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