Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms

Burki Rajendar, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Alloxazine can bind to adenine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-TCC AGX GCA AC-3′/3′-AGG TCN CGT TG-5′, X = AP site, N = A, T, C, G) with a dissociation constant of 0.82 μM (pH 7.0, I = 0.11 M, at 5 °C), and it is applicable to SNPs typing of PCR amplification products based on the binding-induced fluorescence response.

Original languageEnglish
Pages (from-to)670-673
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume6
Issue number4
DOIs
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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