Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles

Akira C. Saito, Tsubasa S. Matsui, Masaaki Sato, Shinji Deguchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Sheets of cells can be used for tissue regenerative medicine. Cell alignment within the sheet is now a key factor in the next generation of this technology. Anisotropic cell sheets without random cell orientations have been conventionally produced with photolithographically, microfabricated substrates using special facilities and equipment. Here we demonstrate a more accessible approach to the fabrication of anisotropic substrates. We locally deformed part of an elastic membrane and simultaneously oxidized the surface to create microwrinkles as well as to enable adhesion to the extracellular matrix. The approach with the local loading made it possible to orient cells in controlled directions within a single membrane sheet depending on the strains determined by the controllable deformation. This technique potentially enables a versatile design of microwrinkles for target-compatible cell alignments.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalBiotechnology Letters
Issue number2
Publication statusPublished - 2014 Feb


  • Cell orientation
  • Cell sheets
  • Contact guidance
  • Microwrinkles
  • Nanotopography
  • Orientation of cells
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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