Agrobacterium tumefaciens increases cytokinin production in plastids by modifying the biosynthetic pathway in the host plant

Hitoshi Sakakibara, Hiroyuki Kasahara, Nanae Ueda, Mikiko Kojima, Kentaro Takei, Shojiro Hishiyama, Tadao Asami, Kazunori Okada, Yuji Kamiya, Tomoyuki Yamaya, Shinjiro Yamaguchi

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    81 Citations (Scopus)

    Abstract

    Agrobacterium tumefaciens infects plants and induces the formation of tumors called "crown galls" by integrating the transferred-DNA (T-DNA) region of the Ti-plasmid into the plant nuclear genome. Tumors are formed because the T-DNA encodes enzymes that modify the synthesis of two plant growth hormones, auxin and cytokinin (CK). Here, we show that a CK biosynthesis enzyme, Tmr, which is encoded by the Agrobacterium T-DNA region, is targeted to and functions in plastids of infected plant cells, despite having no typical plastid-targeting sequence. Evidence is provided that Tmr is an adenosine phosphate-isopentenyltransferase (IPT) that creates a new CK biosynthesis bypass by using 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBDP) as a substrate. Unlike in the conventional CK biosynthesis pathway in plants, trans-zeatin-type CKs are produced directly without the requirement for P450 monooxygenase- mediated hydroxylation. Consistent with the plastid localization of Tmr, HMBDP is an intermediate in the methylerythritol phosphate pathway, a plastid-localized biosynthesis route for universal isoprenoid precursors. These results demonstrate that A. tumefaciens modifies CK biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis.

    Original languageEnglish
    Pages (from-to)9972-9977
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume102
    Issue number28
    DOIs
    Publication statusPublished - 2005 Jul 12

    Keywords

    • Crown gall
    • Isopentenyltransferase
    • Methylerythritol phosphate pathway

    ASJC Scopus subject areas

    • General

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  • Cite this

    Sakakibara, H., Kasahara, H., Ueda, N., Kojima, M., Takei, K., Hishiyama, S., Asami, T., Okada, K., Kamiya, Y., Yamaya, T., & Yamaguchi, S. (2005). Agrobacterium tumefaciens increases cytokinin production in plastids by modifying the biosynthetic pathway in the host plant. Proceedings of the National Academy of Sciences of the United States of America, 102(28), 9972-9977. https://doi.org/10.1073/pnas.0500793102