Aging decreases antioxidant effects and increases lipid peroxidation in the Apolipoprotein E deficient mouse

Taro Honma, Tsuyoshi Tsuduki, Soko Sugawara, Yasuna Kitano, Junya Ito, Ryo Kijima, Mari Tsubata, Kiyotaka Nakagawa, Teruo Miyazawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study, to study the effect of aging and Apolipoprotein E (ApoE) deficiency on antioxidant ability in mice, we examined whether lipid peroxidation is promoted by aging in ApoE deficient (ApoE-/-) mice, which have a shorter lifespan than normal mice. The levels of thiobarbituric acid-reactive substances (TBARS), a biomarker of lipid peroxidation, were measured in plasma and liver in ApoE-/- mice aged 12 weeks (young) and 52 weeks (early stage of senescence). TBARS in plasma and liver were significantly increased by aging. Next, we examined the reasons why lipid peroxidation was promoted by aging, based on measurement of protein and mRNA levels for antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in liver in ApoE-/- mice aged 12 and 52 weeks. The levels of superoxide dismutase 1 and 2 in liver were significantly decreased by aging. The mRNA level of catalase was also significantly decreased and the mRNA levels of superoxide dismutase 1, superoxide dismutase 2 and glutathione peroxidase 1 all showed a tendency to decrease with age. These results suggest that lipid peroxidation is caused by reduction of antioxidant activity with aging and that this promotes senescence and shortens lifespan in ApoE-/- mice.

Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Volume52
Issue number3
DOIs
Publication statusPublished - 2013 May 1

Keywords

  • Aging
  • Apolipoprotein E
  • Lipid peroxidation
  • Senescence
  • TBARS

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

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