Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC

Shogo Taguchi, Keishi Suga, Keita Hayashi, Makoto Yoshimoto, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.

Original languageEnglish
Pages (from-to)403-408
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume175
DOIs
Publication statusPublished - 2019 Mar 1
Externally publishedYes

Keywords

  • Chlorophyll a
  • J-aggregate
  • Light-harvesting
  • Lipid membrane
  • Localization
  • Photo-reduction

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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