Age-related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle

Sachi Tanaka, Kohtaro Miyazawa, Atsuko Kuwano, Kouichi Watanabe, Shyuichi Ohwada, Hisashi Aso, Shigeru Nishida, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

It is well known that the immune system changes with age during development and maturation in Holstein cattle. But age-related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle still remain unclear. The aim of the present study was to investigate comparative changes of leukocytes (granulocytes, monocytes, B cells and T cells) and T cell subsets (CD4+, CD8+, γδ, CD8+γδ and WC1+γδ T cells) in Japanese Black cattle aged 0.5, 1, 2, 6, 18 and 36-41 (adult) months on flow cytometry using specific monoclonal antibodies for the cell surface markers. T cell proportion was approximately 40% in 2-month-old cattle and decreased to 20.6% in adults. In contrast, B cell proportion significantly increased from 7.4% to 28.2% with age. In T cell subsets the percentage of CD4+ T cells significantly increased from 40.5% to 60%, but that of WC1+γδ T cell subset significantly decreased with age. The percentages of CD8+ and CD8+γδ T cells did not change. The present study details the proportional changes in leukocyte and T cell subsets with age in the peripheral blood of Japanese Black cattle and these findings are similar to those described for Holstein cattle.

Original languageEnglish
Pages (from-to)368-374
Number of pages7
JournalAnimal Science Journal
Volume79
Issue number3
DOIs
Publication statusPublished - 2008 Jun

Keywords

  • Aging
  • Japanese Black cattle
  • Leukocytes
  • T cell subsets

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Age-related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle'. Together they form a unique fingerprint.

Cite this