Age-related change of phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide levels in normal human red blood cells

Teruo Miyazawa, Toshihide Suzuki, Kenshiro Fujimoto, Mikio Kinoshita

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

The age-related occurrence of phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH) in normal human red blood cells (RBC) was confirmed by using chemiluminescence detection-high performance liquid chromatography (CL-HPLC). The concentration (mean ± S.D.) for the healthy young adult (22-27 of age, n = 20) was 93 ± 17 pmol PCOOH and 121 ± 20 pmol PEOOH/ml packed RBC, while for the aged adult (56-92 of age, n = 20) the peroxide content was significantly higher, 162 ± 52 pmol PCOOH and 186 ± 40 pmol PEOOH/ml packed RBC. These results indicate that oxidative stress occurs constantly on RBC, even in normal humans, and that the susceptivity significantly increases with age.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalMechanisms of Ageing and Development
Volume86
Issue number3
DOIs
Publication statusPublished - 1996 Mar 29

Keywords

  • Aging
  • Phosphatidylcholine hydroperoxide
  • Phosphatidylethanolamine hydroperoxide
  • Red blood cells

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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