AFM observation of extracellular matrix adsorbed on thermosensitive polymer-films in water

Hiroshi Sunami; Naoko Ido; Masaru Tanaka; Sadaaki Yamamoto; Masatsugu Shimomura

AFM observation of extracellular matrix adsorbed on thermosensitive polymer-films in water

Hiroshi Sunami, Naoko Ido, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura

Research output: Contribution to conference › Paper › peer-review

Abstract

Poly[2-(2-methoxyethoxy)ethyl methacrylate](PMEMA) with lower critical solution temperatures (LCST) of 22°C was prepared by free-radical polymerization of 2-(2-ethoxyethoxy)ethyl acrylate. The protein adsorption behavior was discussed by taking into account the changes in the surface properties of the polymer-films. There were much FBS proteins attached on the PMEMA films above the LCST compared to assays at lower temperature. Adsorption amounts of the proteins on the PMEMA films increased with increasing the temperature. These results showed that FBS protein adsorptions changed in surface properties governed by polymer transitions from hydrophilic to hydrophobic states.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

AFM
Cell adhesion
Scaffold
Thermosensitive polymer
Tissue engineering /

ASJC Scopus subject areas

Engineering(all)

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title = "AFM observation of extracellular matrix adsorbed on thermosensitive polymer-films in water",

abstract = "Poly[2-(2-methoxyethoxy)ethyl methacrylate](PMEMA) with lower critical solution temperatures (LCST) of 22°C was prepared by free-radical polymerization of 2-(2-ethoxyethoxy)ethyl acrylate. The protein adsorption behavior was discussed by taking into account the changes in the surface properties of the polymer-films. There were much FBS proteins attached on the PMEMA films above the LCST compared to assays at lower temperature. Adsorption amounts of the proteins on the PMEMA films increased with increasing the temperature. These results showed that FBS protein adsorptions changed in surface properties governed by polymer transitions from hydrophilic to hydrophobic states.",

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T1 - AFM observation of extracellular matrix adsorbed on thermosensitive polymer-films in water

AU - Sunami, Hiroshi

AU - Ido, Naoko

AU - Tanaka, Masaru

AU - Yamamoto, Sadaaki

AU - Shimomura, Masatsugu

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Poly[2-(2-methoxyethoxy)ethyl methacrylate](PMEMA) with lower critical solution temperatures (LCST) of 22°C was prepared by free-radical polymerization of 2-(2-ethoxyethoxy)ethyl acrylate. The protein adsorption behavior was discussed by taking into account the changes in the surface properties of the polymer-films. There were much FBS proteins attached on the PMEMA films above the LCST compared to assays at lower temperature. Adsorption amounts of the proteins on the PMEMA films increased with increasing the temperature. These results showed that FBS protein adsorptions changed in surface properties governed by polymer transitions from hydrophilic to hydrophobic states.

AB - Poly[2-(2-methoxyethoxy)ethyl methacrylate](PMEMA) with lower critical solution temperatures (LCST) of 22°C was prepared by free-radical polymerization of 2-(2-ethoxyethoxy)ethyl acrylate. The protein adsorption behavior was discussed by taking into account the changes in the surface properties of the polymer-films. There were much FBS proteins attached on the PMEMA films above the LCST compared to assays at lower temperature. Adsorption amounts of the proteins on the PMEMA films increased with increasing the temperature. These results showed that FBS protein adsorptions changed in surface properties governed by polymer transitions from hydrophilic to hydrophobic states.

KW - AFM

KW - Cell adhesion

KW - Scaffold

KW - Thermosensitive polymer

KW - Tissue engineering /

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T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

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