AFM characterization of single strand-specific endonuclease activity on linear DNA.

K. Umemura, F. Nagami, T. Okada, R. Kuroda

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    The specificity of nucleases for nicked and un-nicked double-stranded DNA has been characterized using atomic force microscopy (AFM). We have found that AFM has advantages over the usual macroscopic analyses, such as sucrose gradient centrifugation or electrophoresis, in characterizing nuclease digestion. In particular, short DNA fragments resulting from non-specific digestion were detected and, thus, the true length distribution of digested DNA was revealed. A simple numerical method is proposed to estimate the number of nicked sites per DNA molecule based on AFM images.

    Original languageEnglish
    Pages (from-to)E39
    JournalNucleic acids research
    Volume28
    Issue number9
    DOIs
    Publication statusPublished - 2000 May 1

    ASJC Scopus subject areas

    • Genetics

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