Advantages of zero-loss imaging in transmission electron microtomography

Yukihiro Nishikawa; Takeshi Kaneko; Hideo Nishioka; Toshio Nishi; Hiroshi Jinnai

Advantages of zero-loss imaging in transmission electron microtomography

Yukihiro Nishikawa, Takeshi Kaneko, Hideo Nishioka, Toshio Nishi, Hiroshi Jinnai

Research output: Contribution to conference › Paper › peer-review

Abstract

Transmission electron microtomography (TEMT) is a powerful technique to investigate the three-dimensional structures in nanometer scale, which is based on the transmission electron microscopy (TEM) and computerized tomography (CT). In this study, we demonstrate the advantage of zero-loss imaging based on electron energy loss spectroscopy (EELS) in the quantitative data acquisition for TEMT. Transmission of zero-loss image during tilting the specimen agreed with the calculated transmission under assumption of the increase of effective specimen thickness, which is necessity to obtain the quantitative reconstructions according to the principle of CT. On the other hand, the transmission of unfiltered images was almost constant during tilting. It is concluded that the zero-loss imaging is always required for TEMT.

Original language	English
Pages	3199-3200
Number of pages	2
Publication status	Published - 2005 Dec 1
Externally published	Yes
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sept 20 → 2005 Sept 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

Keywords

3d image analysis
Computer tomography
Electron energy loss spectroscopy
Transmission electron microscopy
Zero-loss imaging

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Advantages of zero-loss imaging in transmission electron microtomography",

abstract = "Transmission electron microtomography (TEMT) is a powerful technique to investigate the three-dimensional structures in nanometer scale, which is based on the transmission electron microscopy (TEM) and computerized tomography (CT). In this study, we demonstrate the advantage of zero-loss imaging based on electron energy loss spectroscopy (EELS) in the quantitative data acquisition for TEMT. Transmission of zero-loss image during tilting the specimen agreed with the calculated transmission under assumption of the increase of effective specimen thickness, which is necessity to obtain the quantitative reconstructions according to the principle of CT. On the other hand, the transmission of unfiltered images was almost constant during tilting. It is concluded that the zero-loss imaging is always required for TEMT.",

keywords = "3d image analysis, Computer tomography, Electron energy loss spectroscopy, Transmission electron microscopy, Zero-loss imaging",

author = "Yukihiro Nishikawa and Takeshi Kaneko and Hideo Nishioka and Toshio Nishi and Hiroshi Jinnai",

year = "2005",

month = dec,

day = "1",

language = "English",

pages = "3199--3200",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

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TY - CONF

T1 - Advantages of zero-loss imaging in transmission electron microtomography

AU - Nishikawa, Yukihiro

AU - Kaneko, Takeshi

AU - Nishioka, Hideo

AU - Nishi, Toshio

AU - Jinnai, Hiroshi

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Transmission electron microtomography (TEMT) is a powerful technique to investigate the three-dimensional structures in nanometer scale, which is based on the transmission electron microscopy (TEM) and computerized tomography (CT). In this study, we demonstrate the advantage of zero-loss imaging based on electron energy loss spectroscopy (EELS) in the quantitative data acquisition for TEMT. Transmission of zero-loss image during tilting the specimen agreed with the calculated transmission under assumption of the increase of effective specimen thickness, which is necessity to obtain the quantitative reconstructions according to the principle of CT. On the other hand, the transmission of unfiltered images was almost constant during tilting. It is concluded that the zero-loss imaging is always required for TEMT.

AB - Transmission electron microtomography (TEMT) is a powerful technique to investigate the three-dimensional structures in nanometer scale, which is based on the transmission electron microscopy (TEM) and computerized tomography (CT). In this study, we demonstrate the advantage of zero-loss imaging based on electron energy loss spectroscopy (EELS) in the quantitative data acquisition for TEMT. Transmission of zero-loss image during tilting the specimen agreed with the calculated transmission under assumption of the increase of effective specimen thickness, which is necessity to obtain the quantitative reconstructions according to the principle of CT. On the other hand, the transmission of unfiltered images was almost constant during tilting. It is concluded that the zero-loss imaging is always required for TEMT.

KW - 3d image analysis

KW - Computer tomography

KW - Electron energy loss spectroscopy

KW - Transmission electron microscopy

KW - Zero-loss imaging

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M3 - Paper

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T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Advantages of zero-loss imaging in transmission electron microtomography

Abstract

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Keywords

ASJC Scopus subject areas

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