TY - JOUR
T1 - Advanced LSI-based amperometric sensor array with light-shielding structure for effective removal of photocurrent and mode selectable function for individual operation of 400 electrodes
AU - Inoue, Kumi Y.
AU - Matsudaira, Masahki
AU - Nakano, Masanori
AU - Ino, Kosuke
AU - Sakamoto, Chika
AU - Kanno, Yusuke
AU - Kubo, Reyushi
AU - Kunikata, Ryota
AU - Kira, Atsushi
AU - Suda, Atsushi
AU - Tsurumi, Ryota
AU - Shioya, Toshihito
AU - Yoshida, Shinya
AU - Muroyama, Masanori
AU - Ishikawa, Tomohiro
AU - Shiku, Hitoshi
AU - Satoh, Shiro
AU - Esashi, Masayoshi
AU - Matsue, Tomokazu
N1 - Publisher Copyright:
© The Royal Society of Chemistry 2015.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2015/2/7
Y1 - 2015/2/7
N2 - We have developed a large-scale integrated (LSI) complementary metal-oxide semiconductor (CMOS)-based amperometric sensor array system called "Bio-LSI" as a platform for electrochemical bio-imaging and multi-point biosensing with 400 measurement points. In this study, we newly developed a Bio-LSI chip with a light-shield structure and a mode-selectable function with the aim of extending the application range of Bio-LSI. The light shield created by the top metal layer of the LSI chip significantly reduces the noise generated by the photocurrent, whose value is less than 1% of the previous Bio-LSI without the light shield. The mode-selectable function enables the individual operation of 400 electrodes in off, electrometer, V1, and V2 mode. The off-mode cuts the electrode from the electric circuit. The electrometer-mode reads out the electrode potential. The V1-mode and the V2-mode set the selected sensor electrode at two different independent voltages and read out the current. We demonstrated the usefulness of the mode-selectable function. First, we displayed a dot picture based on the redox reactions of 2.0 mM ferrocenemethanol at 400 electrodes by applying two different independent voltages using the V1 and V2 modes. Second, we carried out a simultaneous detection of O2 and H2O2 using the V1 and V2 modes. Third, we used the off and V1 modes for the modification of the osmium-polyvinylpyridine gel polymer containing horseradish peroxidase (Os-HRP) at the selected electrodes, which act as sensors for H2O2. These results confirm that the advanced version of Bio-LSI is a promising tool that can be applied to a wide range of analytical fields.
AB - We have developed a large-scale integrated (LSI) complementary metal-oxide semiconductor (CMOS)-based amperometric sensor array system called "Bio-LSI" as a platform for electrochemical bio-imaging and multi-point biosensing with 400 measurement points. In this study, we newly developed a Bio-LSI chip with a light-shield structure and a mode-selectable function with the aim of extending the application range of Bio-LSI. The light shield created by the top metal layer of the LSI chip significantly reduces the noise generated by the photocurrent, whose value is less than 1% of the previous Bio-LSI without the light shield. The mode-selectable function enables the individual operation of 400 electrodes in off, electrometer, V1, and V2 mode. The off-mode cuts the electrode from the electric circuit. The electrometer-mode reads out the electrode potential. The V1-mode and the V2-mode set the selected sensor electrode at two different independent voltages and read out the current. We demonstrated the usefulness of the mode-selectable function. First, we displayed a dot picture based on the redox reactions of 2.0 mM ferrocenemethanol at 400 electrodes by applying two different independent voltages using the V1 and V2 modes. Second, we carried out a simultaneous detection of O2 and H2O2 using the V1 and V2 modes. Third, we used the off and V1 modes for the modification of the osmium-polyvinylpyridine gel polymer containing horseradish peroxidase (Os-HRP) at the selected electrodes, which act as sensors for H2O2. These results confirm that the advanced version of Bio-LSI is a promising tool that can be applied to a wide range of analytical fields.
UR - http://www.scopus.com/inward/record.url?scp=84921657766&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84921657766&partnerID=8YFLogxK
U2 - 10.1039/c4lc01099j
DO - 10.1039/c4lc01099j
M3 - Article
C2 - 25483361
AN - SCOPUS:84921657766
VL - 15
SP - 848
EP - 856
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 3
ER -