TY - JOUR
T1 - Advanced glycation endproducts stimulate mitogen-activated protein kinase and proliferation in rabbit vascular smooth muscle cells
AU - Satoh, Hiroaki
AU - Togo, Masako
AU - Hara, Masumi
AU - Miyata, Toshio
AU - Han, Katsuken
AU - Maekawa, Hisato
AU - Ohno, Minoru
AU - Hashimoto, Yoshiaki
AU - Kurokawa, Kiyoshi
AU - Watanabe, Tsuyoshi
N1 - Funding Information:
This study was supported, in part, by Grants-in-aid from the Ministry of Education, Science and Culture of Japan, the Mitsui Life Social Welfare Foundation (Tokyo, Japan), the Sankyo Foundation of Life Science (Tokyo, Japan), the Uehara Memorial Foundation (Tokyo, Japan), and the Mochida Memorial Foundation for Medical and Pharmaceutical Research (Tokyo, Japan).
PY - 1997/10/9
Y1 - 1997/10/9
N2 - Advanced glycation end products of bovine serum albumin (AGEs-BSA) exhibited biphasic effects on the proliferation of cultured rabbit vascular smooth muscle cells (VSMCs) in terms of [3H]thymidine incorporation and cell number count; a stimulatory effect was observed at 1-10 μg/ml and an inhibitory effect at more than 20 μg/ml, while it inhibited [3H]thymidine incorporation even at 1-10 μg/ml in cultured bovine vascular endothelial cells (VECs). Transient activation of p42 mitogen-activated protein kinase (MAPK) with a peak at around 5 min and a subsequent sustained phase was induced by AGEs-BSA in VSMCs, but not in VECs. The dependence of MAPK activation on AGEs-BSA dose was correlated with that of VSMCs proliferation.
AB - Advanced glycation end products of bovine serum albumin (AGEs-BSA) exhibited biphasic effects on the proliferation of cultured rabbit vascular smooth muscle cells (VSMCs) in terms of [3H]thymidine incorporation and cell number count; a stimulatory effect was observed at 1-10 μg/ml and an inhibitory effect at more than 20 μg/ml, while it inhibited [3H]thymidine incorporation even at 1-10 μg/ml in cultured bovine vascular endothelial cells (VECs). Transient activation of p42 mitogen-activated protein kinase (MAPK) with a peak at around 5 min and a subsequent sustained phase was induced by AGEs-BSA in VSMCs, but not in VECs. The dependence of MAPK activation on AGEs-BSA dose was correlated with that of VSMCs proliferation.
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U2 - 10.1006/bbrc.1997.7424
DO - 10.1006/bbrc.1997.7424
M3 - Article
C2 - 9345279
AN - SCOPUS:0031561417
VL - 239
SP - 111
EP - 115
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -