Advanced glycation endproducts stimulate mitogen-activated protein kinase and proliferation in rabbit vascular smooth muscle cells

Hiroaki Satoh, Masako Togo, Masumi Hara, Toshio Miyata, Katsuken Han, Hisato Maekawa, Minoru Ohno, Yoshiaki Hashimoto, Kiyoshi Kurokawa, Tsuyoshi Watanabe

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Advanced glycation end products of bovine serum albumin (AGEs-BSA) exhibited biphasic effects on the proliferation of cultured rabbit vascular smooth muscle cells (VSMCs) in terms of [3H]thymidine incorporation and cell number count; a stimulatory effect was observed at 1-10 μg/ml and an inhibitory effect at more than 20 μg/ml, while it inhibited [3H]thymidine incorporation even at 1-10 μg/ml in cultured bovine vascular endothelial cells (VECs). Transient activation of p42 mitogen-activated protein kinase (MAPK) with a peak at around 5 min and a subsequent sustained phase was induced by AGEs-BSA in VSMCs, but not in VECs. The dependence of MAPK activation on AGEs-BSA dose was correlated with that of VSMCs proliferation.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalBiochemical and biophysical research communications
Volume239
Issue number1
DOIs
Publication statusPublished - 1997 Oct 9
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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