Abstract
Surface phenotypes and genotypes of six T cell lines established from four patients with adult T cell leukemia (ATL) were compared with those of fresh ATL cells. The surface antigen densities of CD3 and T cell receptor (TCR) complex examined by OKT3 and WT-31 mAbs on fresh ATL cells were low. But three IL-2-dependent cell lines, SKT-2, SKT-5, and ATL-17-2, established from three patients expressed a high density of CD3-TCR complex on their surfaces. On the contrary, three other cell lines (SKT-1A, SKT-1B and MMT-2), established from two other patients, expressed a low density of CD3-TCR complex. Genotypic analysis of TCR β chain (Tβ) gene and integration sites of the proviral genome of human T cell lymphotropic virus type 1 demonstrated that SKT-1A, SKT-1B, and MMT-2 were derived from the original leukemic clones. These apparent associations between a defect of expression of CD3-TCR complex and identical genotypes of fresh and cultured cells suggest that the defect of expression of CD3-TCR complex may play a key role for developement of ATL.
Original language | English |
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Pages (from-to) | 728-733 |
Number of pages | 6 |
Journal | Leukemia |
Volume | 2 |
Issue number | 11 |
Publication status | Published - 1988 |
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research