Adsorption behavior of serum albumin on electrode surfaces and the effects of electrode potential

Bei Guo, Jun Ichi Anzai, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

The adsorption behavior of serum albumin onto the surface of platinum, gold, and glassy carbon electrodes was studied in relation to the electrode potential, by using cyclic voltammetry and a quartz-crystal microbalance. The kinetics of adsorption was significantly dependent on the electrode potential. The adsorption was highly accelerated by the application of positive potential to the electrode, suggesting an electrostatic interaction between the negatively charged albumin molecules and the positively polarized electrodes was irreversible with respect to dilution of the albumin solution, while the albumin molecules were desorbed in part upon shifting the electrode potential in the negative direction. The quartz-crystal microbalance data showed that albumin forms a monomolecular layer on the electrode surface. Protein adsorption on electrode surfaces in serum was also examined.

Original languageEnglish
Pages (from-to)800-803
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume44
Issue number4
DOIs
Publication statusPublished - 1996 Apr

Keywords

  • adsorption
  • cyclic voltammetry
  • electrode
  • quartz-crystal microbalance
  • serum albumin

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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