Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells

Yin Li, Yan Zhang, Shigeki Shibahara, Kazuhiro Takahashi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Expression of adrenomedullin (AM), a potent vasodilator peptide, was studied during adipocyte differentiation of human mesenchymal stem cells (hMSCs). Immunoreactive AM levels in the medium were increased at day 4 and 8 of the adipocyte differentiation. Northern blot analysis showed increased expression of AM mRNA in hMSCs-derived adipocytes at day 4, 8, 12, and 18. Transient transfection assay showed that the promoter activity was higher in hMSCs-derived adipocytes than in hMSCs, when cells were transfected with plasmids containing a cis-acting region (-70/-29) of the human AM gene. Electrophoretic mobility shift assay showed that specific bands bound to the region (-70/-29) in hMSCs-derived adipocytes but not in hMSCs, and were abolished by the stimulatory protein 1 (Sp1) antibody. The present study has shown that AM expression is up-regulated during adipocyte differentiation of hMSCs probably via the interaction between Sp1 or Sp1-related factor(s) and the AM promoter region (-70/-29).

Original languageEnglish
Pages (from-to)616-622
Number of pages7
JournalBiochemical and biophysical research communications
Volume350
Issue number3
DOIs
Publication statusPublished - 2006 Nov 24

Keywords

  • Adipocytes
  • Adrenomedullin
  • Differentiation
  • Expression
  • Gene

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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