TY - JOUR
T1 - Adrenomedullin and its receptor complexes in remnant kidneys of rats with renal mass ablation
T2 - Decreased expression of calcitonin receptor-like receptor and receptor-activity modifying protein-3
AU - Totsune, Kazuhito
AU - Takahashi, Kazuhiro
AU - Mackenzie, Harald S.
AU - Arihara, Zenei
AU - Satoh, Fumitoshi
AU - Sone, Masahiko
AU - Murakami, Osamu
AU - Ito, Sadayoshi
AU - Brenner, Barry M.
AU - Mouri, Toraichi
N1 - Funding Information:
The authors are grateful to Ms Kumi Kikuchi for her technical assistance. This study has been supported in part by a Research Grant from the Takeda Science Foundation and by a Research Grant from the Renal Anemia Research.
PY - 2001
Y1 - 2001
N2 - Adrenomedullin (AM) has vasodilator and diuretic actions, similarly to natriuretic peptides. AM receptor complexes are composed of calcitonin receptor-like receptor (CRLR) and receptor-activity modifying protein-2 (RAMP2), or CRLR and RAMP3. We aimed to know whether gene expression of AM and AM receptor complexes are regulated in kidneys under pathophysiological conditions. Expression of AM, RAMP2, RAMP3 and CRLR mRNA was studied in the remnant kidney of rats with renal mass ablation using competitive quantitative RT-PCR techniques. Partial cloning was performed to determine the rat RAMP3 nucleotide sequence. In normal rat kidneys, expression levels of RAMP2, RAMP3, CRLR and AM mRNAs were 26.5 ± 1.9 mmol/mole of GAPDH, 7.7 ± 0.9 mmol/mole of GAPDH, 3.6 ± 0.2 mmol/mole of GAPDH and 0.57 ± 0.03 mmol/mole of GAPDH (mean ± SE, n = 6), respectively. RAMP3 mRNA levels decreased significantly to about 50% and about 70% of control (sham-operated rats) 4 days and 14 days after 5/6 nephrectomy, respectively. CRLR mRNA levels also decreased significantly to about 30% and about 43% of control. Sodium intake restriction had no significant effects on the RAMP3 and CRLR gene expression. On the other hand, RAMP2 mRNA expression in the kidney was suppressed by sodium intake restriction regardless of nephrectomy, while RAMP2 levels in the remnant kidney were not significantly changed by 5/6 nephrectomy. Neither 5/6 nephrectomy or sodium intake restriction had any significant effects on the AM gene expression in the kidney. The present study showed that expression of mRNAs encoding AM, RAMP2, RAMP3 and CRLR were differentially regulated in remnant kidneys of rats with renal mass ablation.
AB - Adrenomedullin (AM) has vasodilator and diuretic actions, similarly to natriuretic peptides. AM receptor complexes are composed of calcitonin receptor-like receptor (CRLR) and receptor-activity modifying protein-2 (RAMP2), or CRLR and RAMP3. We aimed to know whether gene expression of AM and AM receptor complexes are regulated in kidneys under pathophysiological conditions. Expression of AM, RAMP2, RAMP3 and CRLR mRNA was studied in the remnant kidney of rats with renal mass ablation using competitive quantitative RT-PCR techniques. Partial cloning was performed to determine the rat RAMP3 nucleotide sequence. In normal rat kidneys, expression levels of RAMP2, RAMP3, CRLR and AM mRNAs were 26.5 ± 1.9 mmol/mole of GAPDH, 7.7 ± 0.9 mmol/mole of GAPDH, 3.6 ± 0.2 mmol/mole of GAPDH and 0.57 ± 0.03 mmol/mole of GAPDH (mean ± SE, n = 6), respectively. RAMP3 mRNA levels decreased significantly to about 50% and about 70% of control (sham-operated rats) 4 days and 14 days after 5/6 nephrectomy, respectively. CRLR mRNA levels also decreased significantly to about 30% and about 43% of control. Sodium intake restriction had no significant effects on the RAMP3 and CRLR gene expression. On the other hand, RAMP2 mRNA expression in the kidney was suppressed by sodium intake restriction regardless of nephrectomy, while RAMP2 levels in the remnant kidney were not significantly changed by 5/6 nephrectomy. Neither 5/6 nephrectomy or sodium intake restriction had any significant effects on the AM gene expression in the kidney. The present study showed that expression of mRNAs encoding AM, RAMP2, RAMP3 and CRLR were differentially regulated in remnant kidneys of rats with renal mass ablation.
KW - Adrenomedullin
KW - Calcitonin receptor-like receptor
KW - Kidney
KW - RT-PCR
KW - Receptor-activity modifying protein
KW - Renal failure
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U2 - 10.1016/S0196-9781(01)00526-5
DO - 10.1016/S0196-9781(01)00526-5
M3 - Article
C2 - 11754984
AN - SCOPUS:18244380832
VL - 22
SP - 1933
EP - 1937
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 11
ER -