Adrenomedullin: A possible regulator of germinal vesicle breakdown

Yuuki Hiradate, Jun Ohtake, Yumi Hoshino, Kentaro Tanemura, Eimei Sato

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Adrenomedullin (ADM) is a multifunctional hormone that regulates processes as diverse as blood pressure and cell growth. Although expressed in the ovary, the role of ADM in this organ is not clear. In the present study, we found the expression of ADM receptor and receptor activity-modifying proteins in mouse cumulus cells but not in the oocytes. We report that germinal vesicle breakdown (GVBD), which is required for oocyte maturation, is not inhibited by ADM alone. However, ADM in the presence of the nitric oxide donor sodium nitroprusside (SNP) significantly inhibited GVBD. Furthermore, the ADM- and SNP-dependent inhibition of GVBD was abrogated by Akt blockade. Additionally, Akt expression and phosphorylation was exhibited by ADM, suggesting that Akt signaling upstream in cumulus cells is responsible. Additionally, immunohistochemical analysis revealed that ADM was localized in the granulosa cells of developed follicles, implying the possibility that ADM physiologically affects oocyte maturation in vivo. Our results provide the evidence that ADM can act as a GVBD regulator.

Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalBiochemical and biophysical research communications
Volume415
Issue number4
DOIs
Publication statusPublished - 2011 Dec 2

Keywords

  • Adrenomedullin
  • Akt
  • Cumulus cell
  • GVBD
  • Nitric oxide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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