Adrenal CYP11B1/2 expression in primary aldosteronism: Immunohistochemical analysis using novel monoclonal antibodies

Yasuhiro Nakamura, Takashi Maekawa, Saulo J.A. Felizola, Fumitoshi Satoh, Xin Qi, Carolina Velarde-Miranda, Maria W. Plonczynski, Kazue Ise, Kumi Kikuchi, William E. Rainey, Elise P. Gomez-Sanchez, Celso E. Gomez-Sanchez, Hironobu Sasano

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

CYP11B1 and CYP11B2 play pivotal roles in adrenocorticosteroids synthesis. We performed semi-quantitative immunohistochemical analysis of these proteins in adrenals from patients with primary aldosteronism using novel monoclonal antibodies. Clusters of cortical cells positive for CYP11B2 were detected in the zona glomerulosa (ZG) of normal adrenal gland (NA), idiopathic hyperaldosteronism (IHA) and the adjacent adrenal of aldosterone-producing adenoma (APA). In APA, heterogenous immunolocalization of CYP11B2 and diffuse immunoreactivity of CYP11B1 were detected in tumor cells, respectively. The relative immunoreactivity of CYP11B2 in the ZG of adjacent adrenal of APA was significantly lower than that of NA, IHA and APA tumor cells, suggestive of suppressed aldosterone biosynthesis in these cells. These findings did indicate the regulatory mechanisms of aldosterone biosynthesis were different between normal/hyperplastic and neoplastic aldosterone-producing cells in human adrenals. CYP11B2 immunoreactivity in the ZG could also serve as a potential immunohistochemical marker differentiating morphologically hyperplastic ZG of IHA and APA adjacent adrenal.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume392
Issue number1-2
DOIs
Publication statusPublished - 2014 Jul 5

Keywords

  • APA
  • Adrenal cortex
  • CYP11B1
  • CYP11B2
  • IHA
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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