TY - JOUR
T1 - Adrenal CYP11B1/2 expression in primary aldosteronism
T2 - Immunohistochemical analysis using novel monoclonal antibodies
AU - Nakamura, Yasuhiro
AU - Maekawa, Takashi
AU - Felizola, Saulo J.A.
AU - Satoh, Fumitoshi
AU - Qi, Xin
AU - Velarde-Miranda, Carolina
AU - Plonczynski, Maria W.
AU - Ise, Kazue
AU - Kikuchi, Kumi
AU - Rainey, William E.
AU - Gomez-Sanchez, Elise P.
AU - Gomez-Sanchez, Celso E.
AU - Sasano, Hironobu
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014/7/5
Y1 - 2014/7/5
N2 - CYP11B1 and CYP11B2 play pivotal roles in adrenocorticosteroids synthesis. We performed semi-quantitative immunohistochemical analysis of these proteins in adrenals from patients with primary aldosteronism using novel monoclonal antibodies. Clusters of cortical cells positive for CYP11B2 were detected in the zona glomerulosa (ZG) of normal adrenal gland (NA), idiopathic hyperaldosteronism (IHA) and the adjacent adrenal of aldosterone-producing adenoma (APA). In APA, heterogenous immunolocalization of CYP11B2 and diffuse immunoreactivity of CYP11B1 were detected in tumor cells, respectively. The relative immunoreactivity of CYP11B2 in the ZG of adjacent adrenal of APA was significantly lower than that of NA, IHA and APA tumor cells, suggestive of suppressed aldosterone biosynthesis in these cells. These findings did indicate the regulatory mechanisms of aldosterone biosynthesis were different between normal/hyperplastic and neoplastic aldosterone-producing cells in human adrenals. CYP11B2 immunoreactivity in the ZG could also serve as a potential immunohistochemical marker differentiating morphologically hyperplastic ZG of IHA and APA adjacent adrenal.
AB - CYP11B1 and CYP11B2 play pivotal roles in adrenocorticosteroids synthesis. We performed semi-quantitative immunohistochemical analysis of these proteins in adrenals from patients with primary aldosteronism using novel monoclonal antibodies. Clusters of cortical cells positive for CYP11B2 were detected in the zona glomerulosa (ZG) of normal adrenal gland (NA), idiopathic hyperaldosteronism (IHA) and the adjacent adrenal of aldosterone-producing adenoma (APA). In APA, heterogenous immunolocalization of CYP11B2 and diffuse immunoreactivity of CYP11B1 were detected in tumor cells, respectively. The relative immunoreactivity of CYP11B2 in the ZG of adjacent adrenal of APA was significantly lower than that of NA, IHA and APA tumor cells, suggestive of suppressed aldosterone biosynthesis in these cells. These findings did indicate the regulatory mechanisms of aldosterone biosynthesis were different between normal/hyperplastic and neoplastic aldosterone-producing cells in human adrenals. CYP11B2 immunoreactivity in the ZG could also serve as a potential immunohistochemical marker differentiating morphologically hyperplastic ZG of IHA and APA adjacent adrenal.
KW - APA
KW - Adrenal cortex
KW - CYP11B1
KW - CYP11B2
KW - IHA
KW - Monoclonal antibody
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U2 - 10.1016/j.mce.2014.05.002
DO - 10.1016/j.mce.2014.05.002
M3 - Article
C2 - 24837548
AN - SCOPUS:84901687899
VL - 392
SP - 73
EP - 79
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -