TY - JOUR
T1 - ADP-sensitive purinoceptors induce steroidogenesis via adenylyl cyclase activation in bovine adrenocortical fasciculata cells
AU - Nishi, Haruhisa
AU - Kato, Fusao
AU - Masaki, Eiji
AU - Kawamura, Masahiro
PY - 2002/9
Y1 - 2002/9
N2 - 1. The role of P2Y receptors in the production of cAMP and the activation of protein kinase A (PKA) was studied with respect to the regulation of the steroidogenesis in primary cultures of bovine adrenocortical fasciculata cells (BAFCs). 2. ADP and ATP stimulated cAMP production with EC50 values of 23.7±6.8 μM and 40.1±5.5 μM, respectively. In contrast, the EC50 of BzATP for cAMP production was 153.0±37.4 μM. Adenosine and AMP (0.1-1000 μM) were much less effective than ADP and ATP. 2MeSADP and UTP did not exert detectable effects. ADP (10 and 100 μM) significantly stimulated steroidogenesis; the process was blocked by an adenylyl cyclase inhibitor SQ22536 (100 μM) but not by the P2Y1 receptor antagonist MRS2179 (100 μM). 3. Real-time imaging of the PKA activity with the dye ARII, which became less fluorescent upon phosphorylation, revealed that ADP (100 μM) immediately activated PKA. These effects could be mimicked by forskolin (100 μM) and were blocked by the PKA inhibitor H89 (50 μM). UTP (100 μM) did not activate PKA. 4. The cytoplasm harvested from morphologically and electrophysiologically identified single BAFCs contained mRNA for P2Y2 but not for P2Y1, P2Y4, P2Y11 or P2Y12 receptors, as confirmed by single-cell RT-PCR amplification (50 cycles). 5. These results suggest an expression of an ADP-sensitive Gs-coupled purinoceptor in BAFCs. We propose that this not yet described type of P2Y receptor might mediate the extracellular purine-activated steroidogenesis via cAMP/PKA-mediated pathways, independently from the pathways involving InsP3 production and consequent intracellular Ca2+ increase.
AB - 1. The role of P2Y receptors in the production of cAMP and the activation of protein kinase A (PKA) was studied with respect to the regulation of the steroidogenesis in primary cultures of bovine adrenocortical fasciculata cells (BAFCs). 2. ADP and ATP stimulated cAMP production with EC50 values of 23.7±6.8 μM and 40.1±5.5 μM, respectively. In contrast, the EC50 of BzATP for cAMP production was 153.0±37.4 μM. Adenosine and AMP (0.1-1000 μM) were much less effective than ADP and ATP. 2MeSADP and UTP did not exert detectable effects. ADP (10 and 100 μM) significantly stimulated steroidogenesis; the process was blocked by an adenylyl cyclase inhibitor SQ22536 (100 μM) but not by the P2Y1 receptor antagonist MRS2179 (100 μM). 3. Real-time imaging of the PKA activity with the dye ARII, which became less fluorescent upon phosphorylation, revealed that ADP (100 μM) immediately activated PKA. These effects could be mimicked by forskolin (100 μM) and were blocked by the PKA inhibitor H89 (50 μM). UTP (100 μM) did not activate PKA. 4. The cytoplasm harvested from morphologically and electrophysiologically identified single BAFCs contained mRNA for P2Y2 but not for P2Y1, P2Y4, P2Y11 or P2Y12 receptors, as confirmed by single-cell RT-PCR amplification (50 cycles). 5. These results suggest an expression of an ADP-sensitive Gs-coupled purinoceptor in BAFCs. We propose that this not yet described type of P2Y receptor might mediate the extracellular purine-activated steroidogenesis via cAMP/PKA-mediated pathways, independently from the pathways involving InsP3 production and consequent intracellular Ca2+ increase.
KW - Cortisol
KW - Cyclic AMP dependent protein kinase
KW - P2Y receptor
KW - Steroidogenesis
UR - http://www.scopus.com/inward/record.url?scp=0036739351&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036739351&partnerID=8YFLogxK
U2 - 10.1038/sj.bjp.0704847
DO - 10.1038/sj.bjp.0704847
M3 - Article
C2 - 12208774
AN - SCOPUS:0036739351
VL - 137
SP - 177
EP - 184
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
SN - 0007-1188
IS - 2
ER -