TY - JOUR
T1 - ADMP2 is essential for primitive blood and heart development in Xenopus
AU - Kumano, Gaku
AU - Ezal, Carin
AU - Smith, William C.
N1 - Funding Information:
We thank Enrique Amaya, Jan Christian, Eddy DeRobertis, Paul Krieg, Paul Mead, Jim Smith, Naoto Ueno, Peter Vize, Chris Wright and Len Zon for plasmids, Matthew Kourakis for his help in generating the phylogenetic tree and his helpful comments on the manuscript. This work was supported by a grant from the NIH (HD41434) to WCS.
PY - 2006/11/15
Y1 - 2006/11/15
N2 - We describe here the cloning of a new member of the TGF-β family with similarity to the anti-dorsalizing morphogenetic proteins (ADMPs). This new gene, ADMP2, is expressed in a broad band of mesendoderm cells that appear to include the progenitors of the endoderm and the ventral mesoderm. Antisense morpholino oligonucleotide knockdown of ADMP2 results in near-complete disruption of primitive blood and heart development, while the development of other mesoderm derivatives, including pronephros, muscle and lateral plate is not disrupted. Moreover, the development of the primitive blood in ADMP2 knockdown embryos cannot be rescued by BMP. These results suggests that ADMP2 plays an early role in specifying presumptive ventral mesoderm in the leading edge mesoderm, and that ADMP2 activity may be necessary to respond to BMP signaling in the context of ventral mesoderm induction.
AB - We describe here the cloning of a new member of the TGF-β family with similarity to the anti-dorsalizing morphogenetic proteins (ADMPs). This new gene, ADMP2, is expressed in a broad band of mesendoderm cells that appear to include the progenitors of the endoderm and the ventral mesoderm. Antisense morpholino oligonucleotide knockdown of ADMP2 results in near-complete disruption of primitive blood and heart development, while the development of other mesoderm derivatives, including pronephros, muscle and lateral plate is not disrupted. Moreover, the development of the primitive blood in ADMP2 knockdown embryos cannot be rescued by BMP. These results suggests that ADMP2 plays an early role in specifying presumptive ventral mesoderm in the leading edge mesoderm, and that ADMP2 activity may be necessary to respond to BMP signaling in the context of ventral mesoderm induction.
KW - Blood
KW - Mesoderm
KW - Xenopus
UR - http://www.scopus.com/inward/record.url?scp=33750361485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750361485&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2006.08.010
DO - 10.1016/j.ydbio.2006.08.010
M3 - Article
C2 - 16959239
AN - SCOPUS:33750361485
VL - 299
SP - 411
EP - 423
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -