Adjuvant-induced improvement of glucose intolerance in type 2 diabetic KK-Ay mice through interleukin-1 and tumor necrosis factor-α

Gen Muto, Jo Satoh, Yoshiko Muto, Kazuma Takahashi, Tetsuya Nakazawa, Mikio Sagara, Shuichi Miyaguchi, Masamitsu Fukuzawa, Xiaoling Qiang, Yoshiyuki Sakata, Yumiko Takizawa, Yan Li, Shin ichiro Bando, Toshimune Housai, Akira Tamagawa, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We reported that administration of complete Freund's adjuvant (CFA) improved glucose tolerance test (GTT) results in obese diabetic KK-Ay mice. In this study, we investigated its mechanism. An injection with CFA remarkably improved GTT for more than a week in KK-Ay mice, although insulin response was not changed compared with saline controls. The hypoglycemic effect of insulin was significantly, but partially, potentiated in the CFA-treated mice compared with the controls, suggesting that CFA stimulated insulin-mediated and non-insulin-mediated glucose disposal. Improvement in the GTT with CFA was partially transferable to nontreated mice by peritoneal exudative cells, but not spleen or lymph node cells. Pretreatment with anti-interleukin (IL)-1α and -1β antibodies or anti-tumor necrosis factor (TNF)-α antibody significantly abrogated the improvement in the GTT with CFA. The results indicate that CFA-induced improvement in glucose intolerance in KK-Ay mice was mediated at least by IL-1 and TNF-α. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalClinical Immunology
Volume97
Issue number3
DOIs
Publication statusPublished - 2000

Keywords

  • Complete Freund's adjuvant
  • Cytokines
  • IL-1
  • Insulin resistance
  • KK-Ay mice
  • TNF-α
  • Type 2 diabetes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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