Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling

Noboru Yamaguchi; Toshio Kukita; Yin Ji Li; Noriaki Kamio; Satoshi Fukumoto; Kazuaki Nonaka; Yuzo Ninomiya; Shigemasa Hanazawa; Yoshihisa Yamashita

doi:10.1016/j.febslet.2007.12.037

Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling

Noboru Yamaguchi, Toshio Kukita, Yin Ji Li, Noriaki Kamio, Satoshi Fukumoto, Kazuaki Nonaka, Yuzo Ninomiya, Shigemasa Hanazawa, Yoshihisa Yamashita

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.

Original language	English
Pages (from-to)	451-456
Number of pages	6
Journal	FEBS Letters
Volume	582
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2007.12.037
Publication status	Published - 2008 Feb 6
Externally published	Yes

Keywords

AMP-activated protein kinase
Adiponectin
Calcium signaling
Nuclear factor of activated T cells c1
Osteoclastogenesis
Receptor activator of nuclear factor-κB ligand

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.12.037

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@article{422e3f66a1534b22b0a215798e057cba,

title = "Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling",

abstract = "We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.",

keywords = "AMP-activated protein kinase, Adiponectin, Calcium signaling, Nuclear factor of activated T cells c1, Osteoclastogenesis, Receptor activator of nuclear factor-κB ligand",

author = "Noboru Yamaguchi and Toshio Kukita and Li, {Yin Ji} and Noriaki Kamio and Satoshi Fukumoto and Kazuaki Nonaka and Yuzo Ninomiya and Shigemasa Hanazawa and Yoshihisa Yamashita",

note = "Funding Information: We thank Drs. I. Shimomura and N. Maeda for generously providing mouse globular adiponectin cDNA, and Drs. K. Sanematsu and Y. Murata for their technical expertise. This study was supported by Grant-in-Aid for Scientific Research (C) 17592184 from the Ministry of Education, Science, Sports, and Culture of Japan.",

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doi = "10.1016/j.febslet.2007.12.037",

language = "English",

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T1 - Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling

AU - Yamaguchi, Noboru

AU - Kukita, Toshio

AU - Li, Yin Ji

AU - Kamio, Noriaki

AU - Fukumoto, Satoshi

AU - Nonaka, Kazuaki

AU - Ninomiya, Yuzo

AU - Hanazawa, Shigemasa

AU - Yamashita, Yoshihisa

N1 - Funding Information: We thank Drs. I. Shimomura and N. Maeda for generously providing mouse globular adiponectin cDNA, and Drs. K. Sanematsu and Y. Murata for their technical expertise. This study was supported by Grant-in-Aid for Scientific Research (C) 17592184 from the Ministry of Education, Science, Sports, and Culture of Japan.

PY - 2008/2/6

Y1 - 2008/2/6

N2 - We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.

AB - We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.

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KW - Calcium signaling

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KW - Osteoclastogenesis

KW - Receptor activator of nuclear factor-κB ligand

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Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling

Abstract

Keywords

ASJC Scopus subject areas

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