Adhesive force of human hepatoma HepG2 cells to endothelial cells and expression of E-selectin

Guanbin Song, Toshiro Ohashi, Naoya Sakamoto, Masaaki Sato

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Expression of adhesion molecules may play an important role in the interaction of tumor cells with vascular endothelial cells during tumor invasion and metastasis. In this study, the adhesive force of human hepatoma HepG2 cells to human umbilical vein endothelial cells (HUVECs) was investigated using a micropipette aspiration technique. Expression of an adhesion molecule, E-selectin, was also observed by immunofluorescence, microscopy. In particular, the adhesive force after stimulation of HUVECs with recombinant human interleukin-1β (rhIL-1β) was examined. The results demonstrated that the adhesive force of HepG2 cells to stimulated HUVECs is significantly higher than that of unstimulated control cells, and that immunofluorescence of E-selectin in stimulated HUVECs showed a higher fluorescent intensity compared to control cells. Moreover, addition of monoclonal anti-human E-selectin decreased the adhesive force of HepG2 cells to stimulated HUVECs by 50%. These results suggest that endothelial E-selectin may be a main mediator of carcinoma metastasis of malignant tumor through blood circulation, possibly increasing the adhesive force of human hepatoma HepG2 cells to HUVECs in the early stage of metastases.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalMCB Molecular and Cellular Biomechanics
Volume3
Issue number2
Publication statusPublished - 2006 Jun
Externally publishedYes

Keywords

  • Adhesive force
  • E-selectin
  • Endothelial cells
  • Hepatoma cells
  • Micropipette aspiration

ASJC Scopus subject areas

  • Biophysics
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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