Abstract
We investigated the bioconjugation of proteins on polymer nanoparticles covered with bio-inert phosphorylcholine groups. Poly[2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl polyethyleneglycol methacrylate] was used as an emulsifier and a surface modifier to prepare the poly (L-lactic acid) nanoparticles by a solvent evaporation technique. The diameter and surface potential of the nanoparticles were approximately 260 nm and -5 mV, respectively. We considered that the polymer chain that transformed the conformation by change in pH may be used as a method of controlling the bio-reaction. The enzyme activity in a different pH under the coexistence of poly (glutamic acid) (PGA) and the enzyme was measured. Therefore, the enzyme activity increased in pH 6 in PGA/enzyme mixture system compared with that in pH 7 while the activity was constant in the enzyme single conjugation regardless of the pH change.
Original language | English |
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Pages (from-to) | 785-788 |
Number of pages | 4 |
Journal | Key Engineering Materials |
Volume | 342-343 |
DOIs | |
Publication status | Published - 2007 Jan 1 |
Externally published | Yes |
Keywords
- Bioconjugation
- Cell-targeting diagnosis
- Nanoparticles
- Phospholipid polymer
ASJC Scopus subject areas
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering