Adhesion Behavior of Microorganisms Isolated from Soil on Hydroxyapatite and Other Materials

Masanobu Kamitakahara, Shohei Takahashi, Taishi Yokoi, Chihiro Inoue, Koji Ioku

Research output: Contribution to journalArticle

Abstract

The adhesion behavior of microorganisms on different materials was examined to obtain basic knowledge for designing support materials for microorganisms. The microorganisms were isolated from soil, and their adhesion behavior on hydroxyapatite (HA), carbon-coated HA (Carbon), poly (vinyl chloride) (PVC), and polyurethane (PU) pellets was investigated. The total metabolic activity on and adherence of microorganisms to the tested materials were in the following order: HA > Carbon > PVC > PU. This order was consistent with the extent of hydrophilicity of the materials. Morphological examination and polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis of microorganisms adhered to the materials revealed that the activities and states of microorganisms were affected by the composition of the pellets. PCR-DGGE analysis revealed various species of microorganisms adhered to the HA pellet. HA ceramics are expected to be one of the most suitable materials for supporting microorganisms.

Original languageEnglish
Pages (from-to)984-993
Number of pages10
JournalApplied Biochemistry and Biotechnology
Volume187
Issue number3
DOIs
Publication statusPublished - 2019 Mar 15

Keywords

  • Adhesion
  • Hydrophilicity
  • Hydroxyapatite
  • Microorganisms
  • Support material

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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