Adequacy of mucosal biopsies for evaluation of intestinal cytokine-specific mRNA - Comparative study of RT-PCR in biopsies and isolated cells from normal and inflamed intestine

Kouhei Fukushima, Gail West, Claudio Fiocchi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Endoscopic biopsies are being increasingly utilized to investigate cytokine profiles in normal and diseased intestine. To evaluate the adequacy of mucosal biopsies as a source of cytokine-specific mRNA, we measured their content of interleukin-1β (IL-1β) and interleukin-2 (IL-2) mRNA by reverse transcription-polymerase chain reaction and compared it to that of autologous lamina propria mononuclear cells in control and inflammatory bowel disease-involved specimens. High-quality total RNA was recovered more consistently from cell isolates than from biopsies. Interleukin-1β mRNA was reliably detected in both cell and biopsy samples, whereas IL-2 mRNA was measurable in all lamina propria cells but not always in biopsies. Compared to controls, levels of IL-1β were significantly elevated in Crohn's disease and ulcerative colitis cells and biopsies, and a weak but significant correlation existed between values derived from the two sources. In contrast, only ulcerative colitis cell isolates but not biopsies contained significantly reduced concentrations of IL-2 mRNA compared to those of control and Crohn's disease samples, and no correlation was found between cell and biopsy contents. Widely different levels of cytokine-specific mRNA were present in closely adjacent biopsies, particularly for IL-2. These results suggest that mucosal biopsies are suitable to assess some but not all cytokine-specific mRNA due to variation in RNA recovery, intrinsic level of cytokine gene expression, and substantial variability of cytokine transcripts.

Original languageEnglish
Pages (from-to)1498-1505
Number of pages8
JournalDigestive Diseases and Sciences
Volume40
Issue number7
DOIs
Publication statusPublished - 1995 Jul 1
Externally publishedYes

Keywords

  • Crohn's disease
  • biopsy
  • inflammatory bowel disease
  • interleukin-1β
  • interleukin-2
  • quantitative reverse transcription-polymerase chain reaction
  • ulcerative colitis

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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