Adenovirus-mediated utrophin gene transfer mitigates the dystrophic phenotype of mdx mouse muscles

Rénald Gilbert, Josephine Nalbantoglu, Basil J. Petrof, Satoru Ebihara, Ghiabe Henri Guibinga, Jonathon M. Tinsley, Amine Kamen, Bernard Massie, Kay E. Davies, George Karpati

    Research output: Contribution to journalArticlepeer-review

    92 Citations (Scopus)

    Abstract

    Utrophin is a close homolog of dystrophin, the protein whose mutations cause Duchenne muscular dystrophy (DMD). Utrophin is present at low levels in normal and dystrophic muscle, whereas dystrophin is largely absent in DMD. In such cases, the replacement of dystrophin using a utrophin gene transfer strategy could be more advantageous because utrophin would not be a neoantigen. To establish if adenovirus (AV)-mediated utrophin gene transfer is a possible option for the treatment of DMD, an AV vector expressing a shortened version of utrophin (AdCMV-Utr) was constructed. The effect of utrophin overexpression was investigated following intramuscular injection of this AV into mdx mice, the mouse model of DMD. When the tibialis anterior (TA) muscles of 3- to 5-day-old animals were injected with 5 μl of AdCMV-Utr (7.0 x 1011 virus/ml), an average of 32% of fibers were transduced and the transduction level remained stable for at least 60 days. The presence of utrophin restored the normal histochemical pattern of the dystrophin-associated protein complex at the cell surface and resulted in a reduction in the number of centrally nucleated fibers. The transduced fibers were largely impermeable to the tracer dye Evans blue, suggesting that utrophin protects the surface membrane from breakage. In vitro measurements of the force decline in response to high-stress eccentric contractions demonstrated that the muscles overexpressing utrophin were more resistant to mechanical stress-induced injury. Taken together, these data indicate that AV-mediated utrophin gene transfer can correct various aspects of the dystrophic phenotype. However, a progressive reduction in the number of transduced fibers was observed when the TA muscles of 30- to 45-day-old mice were injected with 25 μl of AdCMV-Utr. This reduction coincides with a humoral response to the AV and transgene, which consists of a hybrid mouse-human cDNA.

    Original languageEnglish
    Pages (from-to)1299-1310
    Number of pages12
    JournalHuman Gene Therapy
    Volume10
    Issue number8
    DOIs
    Publication statusPublished - 1999 May 20

    ASJC Scopus subject areas

    • Molecular Medicine
    • Molecular Biology
    • Genetics

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