Adenosine-induced cardiac gene expression of ischemic murine hearts revealed by cDNA array hybridization

Masanori Asakura, Masafumi Kitakaze, Yasuhiko Sakata, Hiroshi Asanuma, Shoji Sanada, Jyonjoon Kim, Hisakazu Ogida, Yulin Liao, Koichi Node, Seiji Takashima, Michihiko Tada, Masatsugu Hori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Because many endogenous substances, including adenosine, contribute to the pathophysiology of ischemic hearts, the present study was designed to investigate the transcription responses of murine hearts to ischemia with or without administration of an inhibitor of adenosine receptor, 8-sulfophenyltheophylline (8SPT). Sixty minutes after ligation of the proximal site of the left coronary artery with (n=9) or without (n=9) 8SPT, the hearts were excised to obtain mRNA for cDNA array analysis. In 18,376 cDNA, 2 known genes were upregulated over 10-fold, and 11 known genes were upregulated 5.0-9.9-fold. 8SPT reduced the expressed gene to the control levels. Furthermore, 32 unknown genes were also upregulated over 5.0-fold. In contrast, 11 known genes were down-regulated below 0.2-fold, and 64% of the downregulated genes were restored by 8SPT. The 7 unknown genes were downregulated to levels below 0.2-fold. Therefore, it was concluded that the cardiac expression of 24 known and 39 unknown genes was modulated by ischemic stress, and that these genes appeared to be related to the pathophysiology of the ischemic heart because endogenous adenosine modulated their expression.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalCirculation Journal
Volume66
Issue number1
DOIs
Publication statusPublished - 2002 May 13
Externally publishedYes

Keywords

  • Adenosine
  • CDNA array technique
  • Ischemia
  • MRNA
  • Myocardium

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Asakura, M., Kitakaze, M., Sakata, Y., Asanuma, H., Sanada, S., Kim, J., Ogida, H., Liao, Y., Node, K., Takashima, S., Tada, M., & Hori, M. (2002). Adenosine-induced cardiac gene expression of ischemic murine hearts revealed by cDNA array hybridization. Circulation Journal, 66(1), 93-96. https://doi.org/10.1253/circj.66.93