Adenosine as an active constituent of Auricularia auricula for the inhibition of rabbit platelet aggregation

Atsumobu Murata, Nobuaki Oka, Satoko Ohkubo, Yukari Sato, Masako Funamori, Haruhisa Kikuchi, Masa Aki Tanitsu, Kentaro Kogi, Susumu Sato, Takeshi Tadano, Yoshiteru Oshima, Norimichi Nakahata

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2 Citations (Scopus)

Abstract

Auricularia auricula ("Kikurage" in Japan), a kind of mushrooms, has been taken as an ordinary food in East Asia. In the present study, we examined the inhibitory activity of several extracts from Auricularia auricula on rabbit platelet aggregation. We prepared four extracts of Auricularia auricula; water extract, methanol extract, butanol extract from methanol extract (MeOH-BuOH extract) and water extract from methanol extract (MeOH-water extract). Four extracts inhibited ADP-induced platelet aggregation. Since MeOH-BuOH and MeOH-water extracts showed a potent inhibitory activity, both extracts were used for further analysis. MeOH-BuOH and MeOH-water extracts inhibited ADP- or U46619-induced aggregation in a concentration-dependent manner. MeOH-BuOH extract was much more potent than MeOH-water extract. Since adenosine deaminase eliminated the inhibitory effects of both extracts on ADP- or U46619-induced aggregation, they seemed to contain adenosine that inhibits platelet aggregation. In fact, adenosine inhibited ADP- or U46619-induced aggregation in a concentration-dependent manner, and the content of adenosine in each fraction was almost compatible with the inhibitory activity. Furthermore, CGS15943, an adenosine A2A receptor antagonist, reversed the inhibitory effects of both extracts on ADP- or U46619-induced aggregation. These results suggest that Auricularia auricula has anti-platelet activity, and its constituent adenosine mainly exerts its inhibitory activity.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalPharmacometrics
Volume67
Issue number3-4
Publication statusPublished - 2004 Dec 1

Keywords

  • Adenosine
  • Auricularia auricula
  • Platelet aggregation
  • Rabbit platelets

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Murata, A., Oka, N., Ohkubo, S., Sato, Y., Funamori, M., Kikuchi, H., Tanitsu, M. A., Kogi, K., Sato, S., Tadano, T., Oshima, Y., & Nakahata, N. (2004). Adenosine as an active constituent of Auricularia auricula for the inhibition of rabbit platelet aggregation. Pharmacometrics, 67(3-4), 359-364.