Adenine-uridine base pairing at the water-solid-interface

Michael Weisser, Josua Käshammer, Bernhard Menges, Jin Matsumoto, Fumio Nakamura, Kuniharu Ijiro, Masatsugu Shimomura, Silvia Mittler

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    The formation of the base pair adenine-uracil at a water-solid interface, at an immobilized monolayer of adenine disulfide with adenine groups exposed to the very surface, respectively, is shown here. To overcome the steric hindrance of tightly packed adenine groups in a pure adenine thiolate monolayer on gold, the formation of self-assembled monolayers out of a binary mixture of the adenine disulfide and CH3- or OH-terminated thiols are investigated. Electro-chemical investigations, surface plasmon spectroscopy (PSP, plasmon surface polariton), multimode waveguide-PSP- coupling spectroscopy, contact angle measurements, and spontaneous desorption time-of-flight mass spectrometry were used to characterize the monolayers. The specific base pairing was investigated for a variety of monolayer compositions. A specific base pairing was successful for an optimized mixed adenine/OH-terminated thiol monolayer. Nevertheless unspecific binding is a problem.

    Original languageEnglish
    Pages (from-to)87-95
    Number of pages9
    JournalJournal of the American Chemical Society
    Volume122
    Issue number1
    DOIs
    Publication statusPublished - 2000 Jan 12

    ASJC Scopus subject areas

    • Catalysis
    • Chemistry(all)
    • Biochemistry
    • Colloid and Surface Chemistry

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