Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na + transport

Yasuhiro Kato, Akihiro Hazama, Mutsumi Yamagami, Nobuyuki Uozumi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.

Original languageEnglish
Pages (from-to)2291-2293
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number10
DOIs
Publication statusPublished - 2003 Oct
Externally publishedYes

Keywords

  • AtHKT1
  • Membrane protein
  • Sodium
  • Transporter
  • Xenopus laevis oocyte

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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