Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na + transport

Yasuhiro Kato; Akihiro Hazama; Mutsumi Yamagami; Nobuyuki Uozumi

doi:10.1271/bbb.67.2291

Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na ⁺ transport

Yasuhiro Kato, Akihiro Hazama, Mutsumi Yamagami, Nobuyuki Uozumi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Eight transmembrane segments in the Arabidopsis Na⁺ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na⁺ transport in Xenopus ooytes as detected by electrophysiological measurements.

Original language	English
Pages (from-to)	2291-2293
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	10
DOIs	https://doi.org/10.1271/bbb.67.2291
Publication status	Published - 2003 Oct
Externally published	Yes

Keywords

AtHKT1
Membrane protein
Sodium
Transporter
Xenopus laevis oocyte

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.67.2291

Fingerprint Dive into the research topics of 'Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na <sup>+</sup> transport'. Together they form a unique fingerprint.

Cite this

@article{db32870897104060a738162dde893be2,

title = "Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na + transport",

abstract = "Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.",

keywords = "AtHKT1, Membrane protein, Sodium, Transporter, Xenopus laevis oocyte",

author = "Yasuhiro Kato and Akihiro Hazama and Mutsumi Yamagami and Nobuyuki Uozumi",

note = "Funding Information: This work was supported by a grant-in-aid for Center of Excellence (COE) Research (to NU), the 21st Century COE Program (to NU), Grants-in-Aid for Scientiˆc Research (15013225 and 15380070 to NU), the Promotion of Science Research of the Future Program (to NU) and for JSPS Fellows (05504 to YK).",

year = "2003",

month = oct,

doi = "10.1271/bbb.67.2291",

language = "English",

volume = "67",

pages = "2291--2293",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "10",

}

TY - JOUR

T1 - Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na + transport

AU - Kato, Yasuhiro

AU - Hazama, Akihiro

AU - Yamagami, Mutsumi

AU - Uozumi, Nobuyuki

N1 - Funding Information: This work was supported by a grant-in-aid for Center of Excellence (COE) Research (to NU), the 21st Century COE Program (to NU), Grants-in-Aid for Scientiˆc Research (15013225 and 15380070 to NU), the Promotion of Science Research of the Future Program (to NU) and for JSPS Fellows (05504 to YK).

PY - 2003/10

Y1 - 2003/10

N2 - Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.

AB - Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.

KW - AtHKT1

KW - Membrane protein

KW - Sodium

KW - Transporter

KW - Xenopus laevis oocyte

UR - http://www.scopus.com/inward/record.url?scp=3142669357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142669357&partnerID=8YFLogxK

U2 - 10.1271/bbb.67.2291

DO - 10.1271/bbb.67.2291

M3 - Article

C2 - 14586126

AN - SCOPUS:3142669357

VL - 67

SP - 2291

EP - 2293

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 10

ER -