Abstract
Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.
| Original language | English |
|---|---|
| Pages (from-to) | 2291-2293 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 67 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2003 Oct |
| Externally published | Yes |
Keywords
- AtHKT1
- Membrane protein
- Sodium
- Transporter
- Xenopus laevis oocyte
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
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Cite this
Kato, Y., Hazama, A., Yamagami, M., & Uozumi, N. (2003). Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na + transport. Bioscience, Biotechnology and Biochemistry, 67(10), 2291-2293. https://doi.org/10.1271/bbb.67.2291