Adapter-tagged competitive PCR (ATAC-PCR) - A high-throughput quantitative PCR method for microarray validation

Sakae Saito, Ryo Matoba, Kikuya Kato

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Adapter-tagged competitive PCR (ATAC-PCR) is an advanced version of competitive quantitative PCR that is characterized by the addition of unique adapters to cDNA derived from each sample RNA. Using multiple adapters, we can accurately measure the relative expression ratios of many samples, with a calibration curve obtained from internal standards included in the same reaction. ATAC-PCR can identify differences in gene expression as small as twofold, even from very small amounts of sample RNA. This technique is suitable for confirming results obtained with cDNA microarrays or differential display, and it can process more than a thousand of genes per day when used in conjunction with a capillary DNA sequencer.

Original languageEnglish
Pages (from-to)326-331
Number of pages6
JournalMethods
Volume31
Issue number4
DOIs
Publication statusPublished - 2003 Dec
Externally publishedYes

Keywords

  • Adapter-tagged competitive PCR
  • Gene expression profiling
  • Laser capture microdissection

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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