-We have adapted a human monocytic leukemia cell line [THP-lc12( +)] so that it can proliferate in a completely protein-free chemically defined medium of an equal mixture of Dulbecco’s modified Eagle’s minimum essential medium and Ham’s F12. This cell line was designated as THP-lc12( —). When a sufficient number of THP-lc12( —) cells was seeded in culture, the maximum cell density after 6 days of culture was 1 x 106/ml with a doubling time of 30 hr. Transferrin showed a slight stimulative effect on the growth of THP-lc12( —) cells, but insulin did not. The surface profile of THP-lc12( —) was the same as that of THP-lc12( + ), which possessed Mol. Mo5, LeuM3, My9 and Ia-like antigens as well as a large number of Fc receptors. Phagocytic ability with respect to IgG-coated sheep red blood cells was evident in both THP-lc12(+) and THP-lc12( —) cells. A small percentage of THP-lc12( —) cells retained the ability to reduce nitroblue tetrazolium when stimulated with 12-tetradecanoylphorbol-13-acetate. Culture supernatant from THP-lcl2( —) stimulated the incorporation of 3H-thymidine into its own cells, suggesting an autocrine mechanism by which the growth of THP-lc12( —) cells is induced in a protein-free medium.
- leukemia cell line
- protein-free medium
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)