Acute effect of corticosterone on N-methyl-d-aspartate receptor-mediated Ca 2+ elevation in mouse hippocampal slices

Satoru Sato, Hiromi Osanai, Toshihiro Monma, Tokiko Harada, Ayumi Hirano, Minoru Saito, Suguru Kawato

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We examined the rapid effects of corticosterone (CORT) on N-methyl-d-aspartate (NMDA) receptor-mediated Ca 2+ signals in adult mouse hippocampal slices by using Ca 2+ imaging technique. Application of NMDA caused a transient elevation of intracellular Ca 2+ concentration followed by a decay to a plateau within 150s. The 30min preincubation of CORT induced a significant decrease of the peak amplitude of NMDA-induced Ca 2+ elevation in the CA1 region. The rapid effect of CORT was induced at a stress-induced level (0.4-10μM). Because the membrane non-permeable bovine serum albumin-conjugated CORT also induced a similar rapid effect, the rapid effect of CORT might be induced via putative surface CORT receptors. In contrast, CORT induced no significant effects on NMDA-induced Ca 2+ elevation in the dentate gyrus. In the CA3 region, CORT effects were not evaluated, because the marked elevation of NMDA-induced Ca 2+ signals was not observed there.

Original languageEnglish
Pages (from-to)510-513
Number of pages4
JournalBiochemical and biophysical research communications
Volume321
Issue number2
DOIs
Publication statusPublished - 2004 Aug 20
Externally publishedYes

Keywords

  • Acute effect
  • Ca signals
  • Corticosterone
  • Glucocorticoids
  • Hippocampus
  • NMDA receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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