Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish)

Tomohiko Taguchi, Ken Kitajima, Sadako Inoue, Yasuo Inoue, Ji Mao Yang, Harry Schachter, Inka Brockhausen

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

UDP-GlcNAc:GlcNAcβ1→(GlcNAcβ1→2)Manα1-R[GlcNAc to Man]β1→4N-acetylglucosaminyltransferase VI (GnT VI) activity was shown to be present in crude homogenates of Medaka fish (Oryzias latipes) ovaries using UDP-[14C]GlcNAc and synthetic GlcNAcβ1→6 (GlcNAcβ1→2)Manα1→6Glcβ1→octyl as substrates. Characterization of this activity showed a pH optimum at about pH 7.0 and an absolute requirement for divalent cations. The optimum concentration of Mn2+ was at about 25 mM. This finding is the first report on GnT VI activity in fish; the enzyme has previously been described only in avian tissues.

Original languageEnglish
Pages (from-to)533-536
Number of pages4
JournalBiochemical and biophysical research communications
Volume230
Issue number3
DOIs
Publication statusPublished - 1997 Jan 23

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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