Active recombinant C3a of human anaphylatoxin produced in Escherichia coli

Yoshihiro Fukuoka, Akira Yasui, Takehiko Tachibana

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.

Original languageEnglish
Pages (from-to)1131-1138
Number of pages8
JournalBiochemical and biophysical research communications
Volume175
Issue number3
DOIs
Publication statusPublished - 1991 Mar 29
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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