Active recombinant C3a of human anaphylatoxin produced in Escherichia coli

Yoshihiro Fukuoka; Akira Yasui; Takehiko Tachibana

doi:10.1016/0006-291X(91)91683-4

Active recombinant C3a of human anaphylatoxin produced in Escherichia coli

Yoshihiro Fukuoka, Akira Yasui, Takehiko Tachibana

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.

Original language	English
Pages (from-to)	1131-1138
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	175
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)91683-4
Publication status	Published - 1991 Mar 29

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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