Active recombinant C3a of human anaphylatoxin produced in Escherichia coli

Yoshihiro Fukuoka, Akira Yasui, Takehiko Tachibana

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.

    Original languageEnglish
    Pages (from-to)1131-1138
    Number of pages8
    JournalBiochemical and biophysical research communications
    Volume175
    Issue number3
    DOIs
    Publication statusPublished - 1991 Mar 29

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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