TY - JOUR
T1 - Active recombinant C3a of human anaphylatoxin produced in Escherichia coli
AU - Fukuoka, Yoshihiro
AU - Yasui, Akira
AU - Tachibana, Takehiko
N1 - Funding Information:
We thank Dr.N.Hirasawa (Faculty of Pharmaceutical Science, Tohoku University) for his help of ileum assay. We are grateful to Prof.Dr.A.Oikawa (Research Institute for Tuberculosis and Cancer, Tohoku University) for his valuable advice and critical reading of the manuscript. This work was supported by the Ministry of Education, Science and Culture of Japan with the grants A.Y. and T.T.
PY - 1991/3/29
Y1 - 1991/3/29
N2 - DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.
AB - DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.
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U2 - 10.1016/0006-291X(91)91683-4
DO - 10.1016/0006-291X(91)91683-4
M3 - Article
C2 - 2025244
AN - SCOPUS:0025755869
VL - 175
SP - 1131
EP - 1138
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -