Active hyaluronidase 2 expression in the granulation tissue formed in the healing process of equine superficial digital flexor tendonitis

Yohei Yuda, Yoshinori Kasashima, Atsutoshi Kuwano, Kan Sato, Shunji Hattori, Katsuhiko Arai

Research output: Contribution to journalComment/debatepeer-review

2 Citations (Scopus)

Abstract

Upregulation of hyaluronidase 2 (HYAL2), one of somatic hyaluronidase (HAase), was demonstrated in granulation tissue during the healing of equine superficial digital flexor tendon injuries. The activity of HAase was assessed by hyaluronan (HA)-containing gel zymography and in situ zymography using frozen sections obtained from normal and injured tendon tissues. Elevated HAase activity was identified in the extract from the tendinopathic tissues, with lower levels of the activity in normal tendons. In situ zymography using fluorescently-labeled HA demonstrated HAase activity in the granulation tissue formed in the injured region. In addition, in situ hybridization analysis indicated that fibroblastic cells in the granulation tissue of the injured tendon actively expressed HYAL2 but not HYAL1. Quantitative RT-PCR further confirmed a higher level of amplicons corresponding to HYAL2 in tendonitis-derived samples. These results suggest that elevated HYAL2 activity in the granulation tissue could participate in controlling the healing process in equine tendonitis.

Original languageEnglish
Pages (from-to)219-223
Number of pages5
JournalJournal of Veterinary Medical Science
Volume75
Issue number2
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Horse
  • Hyaluronidase
  • Tendonitis
  • Wound healing
  • Zymography

ASJC Scopus subject areas

  • veterinary(all)

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