TY - JOUR
T1 - Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana
AU - Hyodo, Hideki
AU - Yamakawa, Seiyei
AU - Takeda, Yuji
AU - Tsuduki, Masao
AU - Yokota, Akiho
AU - Nishitani, Kazuhiko
AU - Kohchi, Takayuki
N1 - Funding Information:
We are indebted to Dr Yoshibumi Komeda for kindly supplying seeds of the acaulis mutants. We also thank the Arabidopsis Biological Resource Center for supplying other mutant seeds and cDNA library. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (‘Molecular Mechanisms Controlling Multicellular Organization of Plants’) from the Ministry of Education, Sciences, Sports and Culture of Japan, and in part by the Innovative Technology for the Earth Program, ‘Development of Transgenic Plants for Production of Industrial Materials’ from the New Energy and Indus-
PY - 2003/5
Y1 - 2003/5
N2 - Regulation of cell wall structure plays a central role in growth and differentiation in plants. Xyloglucan endotransglucosylase/hydrolases (XTHs) that catalyze the cleavage and molecular grafting of xyloglucan chains function in loosening and rearrangement of the cell wall. We have characterized XTH9, a member of the XTH family that was isolated by systematic differential screening for highly expressed genes in shoot apices in Arabidopsis. In the vegetative phase, XTH9 transcripts accumulate in the shoot apex region. In the reproductive phase, transcript levels in shoot apices increase further, and they also are detected in flower buds, flower stalks and internodes bearing flowers. XTH9 expression levels were reduced markedly in mutants such as acl which are characterized by short internodal cell lengths, but recover at permissive temperatures in the temperature-sensitive acl mutants. Differential expression of XTH9 along the inflorescence stem was also detected in pin1 where no lateral organs are formed. These observations suggest that XTH9 expression is coordinated with plant development, including the differentiation from vegetative and reproductive meristems and cell elongation of the inflorescence stem.
AB - Regulation of cell wall structure plays a central role in growth and differentiation in plants. Xyloglucan endotransglucosylase/hydrolases (XTHs) that catalyze the cleavage and molecular grafting of xyloglucan chains function in loosening and rearrangement of the cell wall. We have characterized XTH9, a member of the XTH family that was isolated by systematic differential screening for highly expressed genes in shoot apices in Arabidopsis. In the vegetative phase, XTH9 transcripts accumulate in the shoot apex region. In the reproductive phase, transcript levels in shoot apices increase further, and they also are detected in flower buds, flower stalks and internodes bearing flowers. XTH9 expression levels were reduced markedly in mutants such as acl which are characterized by short internodal cell lengths, but recover at permissive temperatures in the temperature-sensitive acl mutants. Differential expression of XTH9 along the inflorescence stem was also detected in pin1 where no lateral organs are formed. These observations suggest that XTH9 expression is coordinated with plant development, including the differentiation from vegetative and reproductive meristems and cell elongation of the inflorescence stem.
KW - Arabidopsis thaliana
KW - Cell elongation
KW - Cell wall
KW - Meristem
KW - Xyloglucan endotransglucosylase/hydrolase (XTH)
UR - http://www.scopus.com/inward/record.url?scp=0038574923&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038574923&partnerID=8YFLogxK
U2 - 10.1023/A:1023904217641
DO - 10.1023/A:1023904217641
M3 - Article
C2 - 12856951
AN - SCOPUS:0038574923
VL - 52
SP - 473
EP - 482
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 2
ER -