Abstract
Background: It remains unclear whether estrogen is produced in prostate cancer (PCa) and how it functions in PCa.
Conclusion: A chimeric co-culture method between breast cancer cells and PCaSC revealed the production of active estrogen in PCaSC. High-dose testosterone therapy might introduce a new potential strategy to treat CRPC.
Results: PCaSC metabolized excessive testosterone to estrogen, which activated estrogen receptor in breast cancer cells. Moreover, estrogen synthesized from testosterone in PCaSC regulated the proliferation of PC-3 cell via repression of some unknown growth factors that were secreted from PCaSC.
Materials and Methods: To examine the production of estrogen in PCa cells, the concentration of estrogen in the medium in which LNCaP cells and PCa-derived stromal cells (PCaSC) were co-cultured, was measured by liquid chromatography-mass spectrometry/mass spectrometry (LCMS/ MS), while aromatase (CYP19) mRNA expression was confirmed by real-time polymerase chain reaction (RT-PCR) methods. To verify whether estrogen is synthesized from testosterone in PCaSC functions, PCaSC were co-cultured with breast cancer MCF-7-E10 cells, which were stablytransfected with ERE-GFP, in the presence of testosterone. GFP expression was detected when PCaSCs could synthesize estrogen. The proliferation of PC-3 cells in the presence of PCaSC was determined by cell count.
Original language | English |
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Pages (from-to) | 221-228 |
Number of pages | 8 |
Journal | Anticancer research |
Volume | 35 |
Issue number | 1 |
Publication status | Published - 2015 Jan 1 |
Keywords
- Castration-resistant prostate cancer
- Chimeric coculture
- Estrogen
- Prostate cancer-derived stromal cell
- Testosterone
ASJC Scopus subject areas
- Oncology
- Cancer Research